Nikon Spotlight on Science
Scientists discuss how Nikon microscope systems impact their research.
The advanced Fluorescence Imaging and biophysics group, ICFO-Institute of Photonic Sciences
Professor and Vice Chairman of the Dept. of Cell Biology
Director and Founder of the Center for Biologic Imaging
University of Pittsburgh
Pittsburgh, Pennsylvania, USA
Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University
Professor and Vice-Chairman of the Department of Cellular and Molecular Pharmacology
Investigator, Howard Hughes Medical Institute (HHMI)
The University of California
San Francisco, California, USA
Professor, Department of Physics, University of Illinois at Urbana-Champaign
Group Leader (Royal Society University Research Fellow)
Randall Division of Cell and Molecular Biophysics
King’s College London
London, United Kingdom
Research Institute for Electronic Science
Sapporo, Hokkaido, Japan
Senior Team Leader, Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute
- AZ-C1 Macro Confocal
Assistant Professor, Department of Pharmaceutical Chemistry, Department of Biochemistry and Biophysics, University of California, San Francisco
Institute of Experimental Medicine of the Hungarian Academy of Sciences (IEM HAS)
Professor of Applied Physics, Department of Physics, University of Genoa
Director of the Department of Nanophysics, Istituto Italiano di Tecnologia
So-called iPS cells are attracting considerable interest as pluripotent stem cells that may open up a whole new world of medicine. The Center for iPS Cell Research and Application (CiRA) at Kyoto University is pursuing a wide range of research activities that aim to realize regenerative medicine utilizing iPS cells. The Nikon BioStation CT cell culture observation system is being used in this iPS cell research and is contributing to its efficiency.
We were pleased to have had an opportunity to speak with Masato Nakagawa, who is engaged in iPS cell research at CiRA.
Peer reviewed video journal articles featuring Nikon equipment.
A protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time.
Approaches for monitoring abiotic stress induced spatial and temporal Ca2+ signals in Arabidopsis cells and tissues using the genetically encoded Ca2+ indicators Aequorin or Case12.
The purpose of this article is to demonstrate a method for measuring human embryonic stem cell colony growth using a video bioinformatics method.
Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging
A compartmentalizing microfluidic device for investigating cancer stem cell migration is described. Highly motile cancer cells are isolated to study molecular mechanisms of aggressive infiltration, potentially leading to more effective future therapies.
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.
The mesothelial clearance assay described here takes advantage of fluorescently labeled cells and time-lapse video microscopy to visualize and quantitatively measure the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers.
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
Changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. Intracellular calcium is imaged using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.
Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Methodologies are presented that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.