Market leading inverted microscope capable of taking on the most advanced bioscience imaging protocols.
Scientists have overcome many live cell imaging challenges using advanced techniques such as TIRF, confocal, FRET, photoactivation and microinjection. At the center of it all is the Eclipse Ti-E, a powerful inverted microscope system that provides instant access to all of these techniques plus revolutionary Nikon CFI60 optics. The Eclipse Ti-E offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging.
Stable Time-lapse Imaging with Automatic Focus Correction System
The Ti-E is equipped with a unique Perfect Focus System (PFS) that automatically corrects focus drift in real time during a prolonged period of time-lapse imaging.
Focus drift resulting from a temperature drop when reagents are added is instantaneously corrected and the rapid change of cells can be captured.
The incorporation of the PFS in the nosepiece unit saves space and allows two optical component levels to be attached simultaneously utilizing stratum structure.
Improved Performance in Broader Wavelength Range
By employing the 870nm wavelength for the coverglass interface detection, near-infrared fluorescence dyes including Cy5.5 can be used. Nikon offers two PFS models, one for UV-visible wavelength imaging and one for multiphoton imaging. The multiphoton model can correct for focus drift even when imaging with wavelengths ranging from 880-1300 nm.
Maintaining Focus at Greater Depths
Focus drift can now be corrected in a broader range of Z-axis planes than ever before. Maintaining focus at greater distances from the objective lens and at greater depths within the specimen is also possible.
In addition, PFS's focus drift correction range has been widened, resulting in more reliable and stable data.
Because PFS can maintain focus at greater depths within the specimen, whole images of intersegmental vessels sprouting upward from the dorsal aorta are clearly captured. Shown in the three channels are three different timepoint volumes (red: 0 mins, green: 110 mins, blue: 240 mins).
Specimen: vasculature of a zebrafish embryo (95-186 µm away from the coverslip).
Movie courtesy of: Dr. Robert Fischer, Marine Biological Laboratory
Compatible with Plastic Dishes and Well Plates
In addition to glass bottom dishes, plastic dishes can be used with PFS.* The system is especially suited to high-throughput screening applications that involve multi-well plates.
* for use with specific objective lenses.
High-speed Motorized Control and Acquisition
The operational speeds of motorized components such as the nosepiece, fluorescence filters and stage have been greatly enhanced, allowing high-speed screening image capture during multi-dimensional experiments. Faster device movement and image acquisition reduce overall light exposure and subsequent photo-toxicity, leading to more meaningful data. The digital Controller Hub significantly increases motorized accessory speed by reducing communication overhead time between components, boosting total operation speed.
High-quality Phase Contrast Images using High NA Lenses
The revolutionary external phase contrast unit enables the use of high N.A. non-phase contrast objective lenses for phase contrast imaging by the use of an external phase ring. As this external phase ring is not in the epi-fluorescence light path, users can capture both fluorescence images as well as phase contrast images without compromising on fluorescence tranmission.
Advanced Integration with Peripherals via NIS-Elements Imaging Software
Nikon's comprehensive imaging software NIS-Elements provides an integrated control of the microscope, cameras, components and peripherals. The intuitive GUI and efficient workflow make the programming of automated imaging sequences up to 6D (X, Y, Z, time, wavelength, multipoint) easy to perform.
Ti-LAPP Modular Illumination System
The new Ti-LAPP system provides a wide range of illumination modules that can be flexibly combined to create an imaging system tailored for your research. The modularity of the Ti-LAPP system also provides flexibility when the system configuration needs to be changed, an important feature in core imaging facilities and labs that have changing imaging needs.
The DMD module enables photoactivation and photoconversion of a user-specified pattern and position(s), whereas the conventional FRAP unit only enables photoactivation of a single, manually-positioned spot.
The H-TIRF module automatically adjusts the focus and incident angle of the laser for TIRF observation by monitoring the reflection beam. An even field of TIRF illumination is achieved with a gradation neutral density (ND) filter.
Flexible Module Combination
The Ti-LAPP system’s modularity and flexible configuration capability provide custom imaging solutions for individual research needs. Modules can also be easily exchanged or added to adapt to changing experimental needs, an important feature for labs with evolving research directions and multi-user, core facilities. For example, by adding a second TIRF module to a single-TIRF configuration, users can easily carry out anisotropy experiments and fast, multi-angle TIRF experiments. Adding a photoactivation/conversion module such as the DMD or FRAP module enables tracking of a sub-fraction of a protein population, providing insights into protein behaviors that would otherwise be illusive when imaging the entire population.
Two-tiered Configuration Capability
Taking advantage of the Nikon Ti’s stratum structure, modules can be incorporated as two separate layers with multiple modules per layer. Using a dual layer configuration enables optimal filter configuration for each illumination module. This enables optimal filter selection and improves experimental accuracy whilst maintaining the highest acquisition speeds.
Multiport Design with a Maximum of Five Imaging Ports
Use of an optional back port enables multiple wavelength FRET imaging with multiple cameras. Moreover, by adding an eyepiece tube base unit with a side port, a maximum of five imaging ports* including left, right and bottom ports are available.
(*With Ti-E/B model with bottom port)