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Objective for Clearing Applications

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New long working-distance objective providing ultra-deep imaging with maximum clarity and contrast.

In the field of neuroscience research, there is an ever-increasing need to image deeper into intact brain tissues while maintaining high resolution and clarity. The CFI Plan Apochromat 10XC Glyc was developed to provide deep imaging capabilities for use with a wide variety of clearing techniques. The support of multiple refractive indices allows observation with not only water, oil and glycerin, but also with various optical clearing liquids. This new objective provides broad chromatic aberration correction and high transmission rates by incorporating Nikon’s exclusive Nano Crystal Coat technology. The long-working-distance objective provides clear, high-contrast images deep inside the tissue with its high numerical aperture.

Whole mouse brain imaging with optical clearing method

10xCGlyc-brain-diagram

PFC 

Striatum 

Hippocampus and Amygdala

Optical slices of H-line mouse whole brain after clearing with LUCID-A
Objective: CFI Plan Apochromat 10XC Glyc (NA 0.5, WD 5.5)
Photos courtesy of: Drs. Ryosuke Kawakami and Tomomi Nemoto, Research Institute for Electronic Science, Hokkaido University


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