Microscope objective with the highest numerical aperture, broadest chromatic correction, and a temperature calibrated correction collar to eliminate spherical aberration.
Nikon's CFI Apochromat TIRF Series Objective Lenses have the highest NA available using standard immersion oil and glass coverslips. Thanks to incredibly high Numerical Aperture, when used in TIRF, they provide the most shallow evanescent field possible with oil immersion lenses. Apochromat TIRF objectives provide chromatic correction from 435nm to an incredible 1064nm. In addition, they have terrific IR throughput, facilitating the ability to image and trap single molecules in the same focus plane. These objectives make a terrific choice for multi-photon applications as well.
For the first time, a temperature-change correction ring has been included with the 60X and 100X Apochromat TIRF oil objectives. Users can easily correct temperature induced changes from 23° C (room temperature) to 37° C (physiological temperature) - in the refractive index of the immersion oil that can cause spherical aberration.
Download Objectives Brochure (8.78MB)
| Model | Dimensions | Transmittance | NA | W.D.
(mm) | Cover glass thickness | Correction ring | Observation |
|---|
| CFI Apochromat TIRF 60XC Oil | Diagram | Graph | 1.49 | 0.12 (0.16-0.10)(23℃)
0.11 (0.13-0.07)(37℃) | 0.13-0.19 (23°C)
0.15-0.21 (37°C) | ∨ | BF, DIC, PH**, POL, FL (visible light, UV*) |
| CFI Apochromat TIRF 100XC Oil | Diagram | Graph | 1.49 | 0.12 (0.16-0.10)(23℃)
0.12 (0.15-0.09)(37℃) | 0.13-0.19 (23°C)
0.14-0.20 (37°C) | ∨ | BF, DIC, PH**, POL, FL (visible light, UV*) |
BF: Brightfield
DF: Darkfield
PH: Phase contrast
POL: Simple polarizing
FL: Fluorescence
*Possible but not recommended
**External phase contrast observation is possible with ECLIPSE Ti2-E
