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Nikon Spotlight on Science

Researcher Interviews

Scientists discuss how Nikon microscope systems impact their research.

Dr. Tadahiro Iimura

Dr. Tadahiro Iimura

Division of Bio-Imaging, Proteo-Science Center (PROS), Ehime University 
Division of Analytical Bio-Medicine, Advanced Research Support Center (ADRES), Ehime University 
Graduate School of Medicine, Ehime University

Melike Lakadamyali, Ph.D.

Melike Lakadamyali, Ph.D.

The advanced Fluorescence Imaging and biophysics group, ICFO-Institute of Photonic Sciences

Nikon Equipment:

Simon C. Watkins, Ph.D.

Simon C. Watkins, Ph.D.

Professor and Vice Chairman of the Dept. of Cell Biology
Director and Founder of the Center for Biologic Imaging
University of Pittsburgh
Pittsburgh, Pennsylvania, USA

Ikuo Wada, Ph.D.

Ikuo Wada, Ph.D.

Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University

Ronald D. Vale, Ph.D.

Ronald D. Vale, Ph.D.

Professor and Vice-Chairman of the Department of Cellular and Molecular Pharmacology
Investigator, Howard Hughes Medical Institute (HHMI)
The University of California
San Francisco, California, USA

Paul Ronald Selvin, Ph.D.

Paul Ronald Selvin, Ph.D.

Professor, Department of Physics, University of Illinois at Urbana-Champaign

Maddy Parsons, Ph.D.

Maddy Parsons, Ph.D.

Group Leader (Royal Society University Research Fellow)
Randall Division of Cell and Molecular Biophysics
King’s College London
London, United Kingdom

Tomomi Nemoto, Ph.D. and Ryosuke Kawakami, Ph.D.

Tomomi Nemoto, Ph.D. and Ryosuke Kawakami, Ph.D.

Research Institute for Electronic Science
Sapporo, Hokkaido, Japan

Nikon Equipment:

Atsushi Miyawaki, M.D., Ph.D.

Atsushi Miyawaki, M.D., Ph.D.

Senior Team Leader, Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute

Nikon Equipment:

  • AZ-C1 Macro Confocal

Bo Huang, Ph.D.

Bo Huang, Ph.D.

Assistant Professor, Department of Pharmaceutical Chemistry, Department of Biochemistry and Biophysics, University of California, San Francisco

Nikon Equipment:

Tamas Freund, Ph.D. and Istvan Katona, Ph.D.

Tamas Freund, Ph.D. and Istvan Katona, Ph.D.

Institute of Experimental Medicine of the Hungarian Academy of Sciences (IEM HAS)
Budapest, Hungary

Nikon Equipment:

Alberto Diaspro, Ph.D.

Alberto Diaspro, Ph.D.

Professor of Applied Physics, Department of Physics, University of Genoa
Director of the Department of Nanophysics, Istituto Italiano di Tecnologia

Prof. Igor Kireev, Ph.D.

Prof. Igor Kireev, Ph.D.

Igor Kireev Ph.D. is the head of imaging core facility at A.N.Belozersky Institute of Physico-chemical biology of Lomonosov Moscow State University

Dr Erik Manders

Dr Erik Manders

Dr Erik Manders is assistant professor and operational manager of the Van Leeuwenhoek centre for advance microscopy at the Universiteit van Amsterdam.

Dr. Jacopo Lucci

Dr. Jacopo Lucci

Chief Scientific Officer at Natural Bio-Medicine SpA,
Aboca Group

Nikon Equipment:

Dr. Arne Seitz, Dr. Romain Guiet and Thierry Laroche

Dr. Arne Seitz, Dr. Romain Guiet and Thierry Laroche

The Faculty of Life Science (SV) at the École Polytechnique Fédéral de Laussane (EPFL), Switzerland, has a long record of excellence in research applied to life sciences

Masato Nakagawa

Masato Nakagawa

So-called iPS cells are attracting considerable interest as pluripotent stem cells that may open up a whole new world of medicine. The Center for iPS Cell Research and Application (CiRA) at Kyoto University is pursuing a wide range of research activities that aim to realize regenerative medicine utilizing iPS cells. The Nikon BioStation CT cell culture observation system is being used in this iPS cell research and is contributing to its efficiency.

We were pleased to have had an opportunity to speak with Masato Nakagawa, who is engaged in iPS cell research at CiRA.

Nikon Equipment:

Prof. Heinz Beck, Ph.D.

Prof. Heinz Beck Ph.D.

Full time professor at the Laboratory of Experimental Epileptology and Cognition Research hosted in the Life & Brain Center, Part of the University of Bonn.

Nikon Equipment:

Prof. Staffan Strömblad, Ph.D.

Prof. Staffan Strömblad, Ph.D.

Staffan Strömblad Ph.D. is group leader at the prestigious Karolinska Institutet, Stockholm, Sweden, an institution that awards the Physiology Nobel Prize yearly. He is also the head of the Live Cell Imaging Facility (LCI), in which the Nikon Center of Excellence for live cell imaging is integrate.

Video Articles

Peer reviewed video journal articles featuring Nikon equipment.

An Ex Vivo Laser-induced Spinal Cord Injury Model

An Ex Vivo Laser-induced Spinal Cord Injury Model

A protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time.

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Approaches for monitoring abiotic stress induced spatial and temporal Ca2+ signals in Arabidopsis cells and tissues using the genetically encoded Ca2+ indicators Aequorin or Case12.

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth

The purpose of this article is to demonstrate a method for measuring human embryonic stem cell colony growth using a video bioinformatics method.

Nikon Equipment:

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

A compartmentalizing microfluidic device for investigating cancer stem cell migration is described. Highly motile cancer cells are isolated to study molecular mechanisms of aggressive infiltration, potentially leading to more effective future therapies.

Nikon Equipment:

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.

Nikon Equipment:

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

The mesothelial clearance assay described here takes advantage of fluorescently labeled cells and time-lapse video microscopy to visualize and quantitatively measure the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers.

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. Intracellular calcium is imaged using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.

Nikon Equipment:

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Methodologies are presented that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.

Nikon Equipment:


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