A1R HD
High Definition Resonant Scanning Confocal
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Equipped with both ultrafast resonant and high-resolution galvano scanners, A1R HD allows simultaneous photoactivation and imaging.
Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, A1R HD is a powerful tool for the acquisition of intracellular dynamics and interaction.
Key Features
New High Definition 1K Resonant Scanner
Nikon’s new resonant scanner mounted in the A1R HD scan head supports both high speed and high definition imaging. The wide dynamic range and reduced noise level raises the bar for image quality in resonant scanners.
High definition
A new resonant scanner achieves finely detailed images with a maximum resolution of 1024 x 1024 pixels (15 fps). A newly developed sampling method produces sharper images with any configuration: even at lower resolution settings. When combined with Nikon’s high NA objective lenses, the A1R HD can achieve absolute optical precision.
Large field of view
With both 1024 x 1024 pixel resolution and a large field of view (FOV18), the new resonant scanner delivers higher throughput in various imaging applications.
High speed
The fast acquisition speed of the resonant scanner (up to 420 fps depending on scan area) is able to capture images with a very short dwell time, minimizing excitation time and light energy exposure of the samples.
Multicolor
Up to 5 channel (four-channel episcopic detector plus diascopic detector) simultaneous imaging is possible.
Zebrafish heart and blood cells imaged with HD resonant scanner. Sample courtesy of Martha Marvin, Ph.D., Williams College
Hybrid Scanner for Ultrafast Photoactivation Imaging
A1R HD has a hybrid scan head that incorporates both an ultrahigh-speed resonant scanner and a high-resolution galvano scanner. Simultaneous photoactivation and ultrafast imaging using these two scanners allow acquisition of rapid changes after photoactivation and enables observation of intermolecular interaction.
What is a hybrid scan head?
This mechanism allows flexible switching or simultaneous use of two scanners (resonant and galvano) with the use of a hyper selector.
High-Resolution Imaging with a Galvano Scanner
The A1R HD's galvano scanner enables high-resolution imaging of up to 4096 x 4096 pixels. In addition, with its scanner driving and sampling systems, plus image correction technology, high-speed acquisition of 10 fps (512 x 512 pixels) is also possible.
Increased light detection efficiency realizes high image quality
The low-angle incidence method utilized on the dichroic mirrors increases fluorescence efficiency by 30%.
Conventional 45° incidence angle method Reflection-transmission characteristics have high polarization dependence
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Low-angle incidence method Reflection-transmission characteristics have lower polarization dependence
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By employing the hexagonal pinhole, higher brightness equivalent to that of a circular pinhole is achieved.
64% of the area of the circle
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30% more light |
83% of the area of the circle
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Nikon's original dual integration signal processing technology (DISP) has been implemented in the image processing circuitry to improve electrical efficiency, resulting in an extremely high S/N ratio.
Fast and Accurate Spectral Imaging: A1-DUS Spectral Detector Unit
High-speed spectral imaging
Acquisition of a 32-channel spectral image (512 x 512 pixels) with a single scan in 0.6 second is possible. Moreover, 512 x 32-pixel images can be captured at 24 fps.
Accurate, high-speed unmixing
Accurate spectral unmixing provides maximum performance in the separation of closely overlapping fluorescence spectra and the elimination of autofluorescence. Superior algorithms and high-speed data processing enable real time unmixing during image acquisition.
Actin of HeLa cell expressing H2B-YFP was stained with Phalloidin-Alexa Fluor® 488.
Spectral image in the 500-692 nm range captured with 488 nm laser excitation
Left: Spectral image, Right: Unmixed image (green: Alexa Fluor® 488, red: YFP)
Specimen courtesy of: Dr. Yoshihiro Yoneda and Dr. Takuya Saiwaki, Faculty of Medicine, Osaka University
Wide band spectral imaging
Simultaneous excitation with four lasers selected from a maximum of eight wavelengths is available, enabling spectral imaging across wider bands.
V-filtering function
Filter-less intensity adjustment is possible by selecting desired spectral ranges from 32 channels that match the spectrum of the fluorescence probe in use and combining them to perform the filtering function.
Bright Spectral Imaging: A1-DUVB GaAsP Detector Unit
High-sensitivity spectral image acquisition
With a GaAsP PMT, the A1-DUVB tunable emission detector delivers flexible detection of fluorescent signals with higher sensitivity, in both the galvano and resonant imaging modalities.
Variable acquisition wavelength range
User-defined emission bands can collect images within a selected wavelength range, replacing the need for fixed bandwidth emission filters.
Users can define the emission bandwidth range to as little as 10nm. Spectral images of multi-labeled specimens can be acquired by capturing a series of spectral images while changing detection wavelengths.
Based on the application, virtual bandpass mode and continuous bandpass modalities are selectable on the A1-DUVB.
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HeLa cells labeled with five-color fluorescence, Nucleus: DAPI, Vimentin: Alexa Fluor® 488, Lamin: Alexa Fluor® 568, Tubulin: Alexa Fluor® 594, Actin: Alexa Fluor® 633
Specimen courtesy of: Dr. Tadashi Karashima, Department of Dermatology, Kurume University School of Medicine
Optional second channel detector
An optional second GaAsP PMT provides flexibility in detection. Users can divert selected wavelengths to the second fixed bandwidth emission channel by inserting a dichroic mirror, while simultaneously utilizing the user-definable emission band on the first channel. The second detector allows FRET, ratio imaging and other applications requiring simultaneous multi-channel imaging.
Accurate spectral unmixing
Multi-channel images acquired with the A1-DUVB can be spectrally unmixed by using the spectra of reference samples, or the spectra within the acquired images.
Simple Operation of Complex Applications
NIS-Elements C control software enables integrated control of the confocal imaging system, microscope and peripheral devices with a simple and intuitive interface. Diverse reliable analysis functions are also available.
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System integration of Ti2-E inverted microscope for multi-mode imaging is possible by equipping the confocal microscope system with N-SIM/N-STORM super resolution microscope system, TIRF system, spectral detector and Perfect Focus System. All systems can be controlled by a single NIS-Elements platform.