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Objective for Clearing Applications

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Designed for imaging whole, cleared tissues providing ultra-deep, large-scale images with maximum clarity and contrast.

The CFI90 20XC Glyc is suitable for deep imaging of tissues and whole organs treated with a variety of tissue clearing methods. The objective features a correction collar that is compatible with a wide range of immersion media and refractive indices ranging from 1.44 to 1.50. With its high NA, large field-of-view, and ultra-long working distance, this objective enables observation of large samples and whole organs with exceptional clarity and throughput. The objective provides superior chromatic aberration correction and transmittance in the 700 nm to 1300 nm wavelength range to provide exceptional images for multiphoton applications.

Sample image acquired with the CFI90 20XC Glyc objective


Key Features

Large Field-of-View and High Resolution

The CFI90 20XC Glyc achieves a unique parfocal distance of 90mm, resulting in both a large field-of-view and high numerical aperture.  This combination enables high-throughput imaging with exceptionally bright, high-resolution images.

Superior Chromatic Aberration Correction and High Transmittance

Chromatic aberration correction in the near-infrared range (700 nm to 1300 nm)  accommodates multiphoton applications. In addition, the lens utilizes Nikon Nano Crystal Coat technology to provide high transmittance and superior signal collection from deep inside whole tissues.

Supports Observation of Large Samples

An ultra-long working distance (WD) of 8.2 mm accommodates large samples such as whole brains.

Compatible with a Wide Range of Tissue Clearing Agents with Different Refractive Indices

The new objective lens is compatible with a wide range of immersion media and tissue clearing agents.  The correction collar accommodates a broad range of refractive indices (1.44-1.50) and corrects spherical aberrations caused by observation depth and temperature changes to provide bright, clear images throughout the entire specimen. 

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