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Confocal Microscope System

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Specifications

Compatible Laser: Solid-state laser: 405 nm, 440 (445) nm, 488 nm, 561 (594) nm, 638 (640) nmAr laser (457 nm/488 nm/514 nm), HeNe laser (543 nm)Solid-state laser: 405 nm, 440 (445) nm, 488 nm, 561 (594) nm, 638 (640) nmAr laser (457 nm/488 nm/514 nm), HeNe laser (543 nm)
Laser Unit: LU-N3: installed laser 405 nm, 488 nm, and 561 nm
*Cannot be used with C2-DUS spectral detector
LU-N4/N4S: installed laser 405 nm, 488 nm, 561 nm, and 640 nm
*Use LU-N4S when using C2-DUS spectral detector
LU-NV series: mountable laser 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm and 647 nm
Standard Detector: Wavelength: 400-750nm
Detector: 3 PMT
Filter cube: 2 filter cubes
Diascopic Detector (option): Wavelength: 400-700nm
Detector: 1 PMT
Spectral Detector (option): C2-DUS spectral detector unit
Number of channels: 32, Wavelength detection range: 400 - 750 nm, Spectral image acquisition speed: 2 fps (256 x 256 pixels), Maximum pixel size: 1024 x 1024 (Spectral mode/Virtual filter mode), Wavelength resolution: 2.5/5.0/10.0 nm, Wavelength range variable in 0.25 nm steps 
C2-DUVB GaAsP detector unit
Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT with a user-defined dichroic mirror and barrier filter, Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest: 320 nm, Maximum pixel size: 2048 x 2048 (CB mode/VB mode), Wavelength resolution: 10 nm, Wavelength range variable in 1 nm steps
Scanning:

Scanning range: square inscribed in a ø18mm circle

With 3 channel fluorescent detector:
Pixel size: max. 2048 x2048 pixels
Scanning speed
Standard mode: 2 fps (512 x 512 pixels, bi-direction), 17 fps (512 x 32 pixels bi-direction), Zoom 1-1000x
Fast mode : 8 fps (512 x 512 pixels, bi-direction), 100fps (512 x 32 pixels, bi-direction)*1

With spectral detector:
Pixel size: max. 1024 x 1024 pixels
Scanning speed: standard 0.5 fps (512 x 512 pixels)
max. 6 fps (64 x 64 pixels)

Scanning Mode: X-Y, X-Z, XY rotation, zoom, ROI, XYZ, time lapse, stimulation, multipoint, image stitching (large image)
Pinhole: Circular shape, 6 size
Image Bit Depth: 12 bits
Compatible Microscopes: ECLIPSE Ti-E/Ti-U inverted microscope
ECLIPSE Ni-E (focusing nosepiece type/stage focusing type)
Ni-U upright microscope
ECLIPSE FN1 fixed stage microscope
AZ100 multi-purpose zoom microscope
Z Step: Ti-E: 0.025 μm, FN1 stepping motor: 0.05 μm, Ni-E: 0.025 μm
NIS-Elements C Software:

Acquisition/Visualization/Image Processing/Analysis:
3D volume/orthogonal viewing and rendering, large image stitching, multi-point timelapse, spectral unmixing, DAQ and I|O control, timelapse analysis, deconvolution, ratio analysis, morphology segmentation, AVI and MOV file output

Applications:
FRAP, FLIP, FRET, photo activation, colocalization, three-dimensional time-lapse imaging, multipoint time-lapse imaging

*1 Fast mode is compatible with zoom 8-1000x. It is not compatible with Rotation, Crop, ROI, Spectral Imaging and Stimulation.


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