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Confocal Microscope System

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Specifications

Scan head input/output port 2 laser input ports
3 signal output ports for standard, spectral and optional detector *1

 

Laser

LU-N3 3-laser unit  405 nm, 488 nm, 561nm lasers are installed ; built-in AOTF *Cannot be used with spectral detector
LU-N4/LU-N4S
 4-laser unit
405 nm, 488 nm, 561nm,640nm laser are installed;built-in AOTF *Use LU-N4S when using the spectral detector
LU-NV series laser unit Compatible lasers : 405 nm, 445 nm, 458nm, 488nm, 514nm, 532nm, 561nm, 594nm, 640nm, 647nm; built-in AOTF
Standard fluorescence detector Wavelength 400-750 nm
Detector A1-DU4 4 Detector Unit: 4 standard PMTs A1-DUG GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 standard PMTs
Filter cube 6 filter cubes commonly used for a microscope mountable on each of three filter wheels Recommended wavelengths: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75
Diascopic detector (option) Wavelength 485-650 nm
Detector PMT
FOV Square inscribed in a ø18 mm circle
Image bit depth 4096 gray intensity levels (12 bit)
Scan head Standard image acquisition Scanner: galvano scanner x2 Pixel size: max. 4096 x 4096 pixels Scanning speed: Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction) Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*2 Zoom: 1-1000x continuously variable Scanning mode: X-Y, X-T, X-Z, XY rotation, Free line
High-speed image acquisition
Dichroic mirror Low-angle incidence method, Position: 8 Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80 Optional filter: 457/514/561
Pinhole 12-256 µm variable (1st image plane)
Spectral detector*3
 (option)
Number of channels 32 channels
Wavelength detection range 400-750 nm
Spectral image acquisition speed 4 fps (256 x 256 pixels), 1000 lps Pixel size: max. 2048 x 2048
Wavelength resolution 80 nm (2.5 nm), 192 nm (6 nm), 320 nm (10 nm) Wavelength range variable in 0.25 nm steps
Unmixing High-speed unmixing, Precision unmixing
Z step Ti-E: 0.025 µm, FN1 stepping motor: 0.05 µm Ni-E: 0.025 µm
Compatible microscopes ECLIPSE Ti-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type)
Option Motorized XY stage (for Ti-E/Ni-E), High-speed Z stage (for Ti-E), High-speed piezo objective-positioning system (for FN1/Ni-E)
Software Display/image generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Image format JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS
Application FRAP, FLIP, FRET(option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control computer OS Microsoft Windows® 7 Professional 64bits SP1
CPU Intel Xeon E5-2643v3 (3.40 GHz/20 MB) or higher
Memory 16 GB or higher
Hard disk 300 GB SAS (15,000 rpm) x2, RAID 0 configuration
Data transfer Dedicated data transfer I/F
Network interface 10/100/1000 Gigabit Ethernet x2
Monitor 1600 x 1200 or higher resolution, dual monitor configuration recommended
Recommended installation conditions Temperature 23 ± 5 ºC, humidity 70 % (RH) or less (non-condensing)

* 1 FCS/FCCS/FLIM is possible in combination with third-party systems

* 2 Fast mode is compatible  with  zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line,  CROP, ROI, Spectral  imaging, Stimulation and FLIM.

* 3 Compatible with galvano scanner only.


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