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Resonant Scanning Confocal System

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Specifications

Scan head input/output port

2 laser input ports

3 signal output ports for standard, spectral and optional detector*1

Laser LU-N3 3-laser unit

405 nm, 488 nm, 561nm lasers are installed; built-in AOTF

* Cannot be used with A1-DUS spectral detector

LU-N4/N4S 4-laser unit

405 nm, 488 nm, 561 nm,640 nm lasers are installed; built-in AOTF

* Use LU-N4S when using A1-DUS spectral detector

LU-NV series laser unit Compatible lasers : 405 nm, 445 nm, 458 nm, 488 nm, 514 nm, 532 nm, 561 nm, 594 nm, 640 nm, 647 nm ; built-in AOTF
Standard fluorescence detector Wavelength 400-750 nm
Detector

A1-DU4 4 Detector Unit: 4 normal PMTs

A1-DUG GaAsP Multi Detector Unit: 2 GaAsP PMTs + 2 normal PMTs

Filter cube

6 filter cubes commonly used for a microscope mountable on each of three filter wheels

Recommended wavelengths: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75

Diascopic detector (Option) Wavelength 485-650 nm
Detector PMT
FOV Square inscribed in a Ø 18 mm circle
Image bit depth 4096 gray intensity levels (12 bit)
Scan head Standard image acquisition

Scanner: galvano scanner x2

Pixel size: max. 4096 x 4096 pixels

Scanning speed:

Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction)

Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*2

Zoom: 1-1000x continuously variable

Scan mode: X-Y, X-T, X-Z, XY rotation, Free line, Line-Z

High-speed image acquisition Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis)
 
Pixel size: max. 512 x 512 pixels
 
Scanning speed: 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed)
 
Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
 
Scan mode: X-Y, X-T, X-Z Acquisition method: Standard image acquisition, High-speed image acquisition, Simultaneous photoactivation and image acquisition
Dichroic mirror

Low-angle incidence method, Number of positions: 8

Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80

Optional filter: 457/514/561

Pinhole 12-256 µm variable (1st image plane)
Spectral detector (option) A1-DUS spectral detector unit

Number of channels: 32

Wavelength detection range: 400 - 750 nm

Spectral image acquisition speed: 4 fps (256 x 256 pixels)

Maximum pixel size: 2048 x 2048 (Spectral mode/Virtual filter mode)

Wavelength resolution: 2.5/6.0/10.0 nm, wavelength range variable in 0.25 nm steps

Compatible with galvano scanner only

A1-DUVB GaAsP detector unit

Number of channels: 1 GaAsP PMT with variable emission plus 1 optional GaAsP PMT (A1-DUVB-OP) with a user-defined dichroic mirror and barrier filter

Wavelength detection range: 400 - 720 nm, narrowest: 10 nm, broadest:320 nm

Maximum pixel size: 4096 x 4096 (CB mode/VB mode)

Wavelength resolution: 10 nm, wavelength range variable in 1 nm steps

Compatible with galvano and resonant scanners

Z step

Ti2-E: 0.02 µm, FN1 stepping motor: 0.05 µm

Ni-E: 0.025 µm

Compatible microscopes ECLIPSE Ti2-E inverted microscope, ECLIPSE FN1 fixed stage microscope, ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type)
Option Motorized XY stage (for Ti2-E/Ni-E), High-speed Z stage (for Ti2-E), High-speed piezo objective-positioning system (for FN1/Ni-E)
Software Display/image generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Image format JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS
Application FRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control computer OS Microsoft Windows® 7 Professional 64bits SP1
CPU Intel Xeon E5-2643v3 (3.40 GHz/20 MB) or higher
Memory 16 GB or higher
Hard disk 300 GB SAS (15,000 rpm) x2, RAID 0 configuration
Data transfer Dedicated data transfer I/F
Network interface Gigabit Ethernet x2
Monitor 1600 x 1200 or higher resolution, dual monitor configuration recommended
Recommended installation conditions Temperature 23 ± 5 ºC, humidity 70 % (RH) or less (non-condensing)

*1FCS/FCCS/FLIM is possible in combination with third-party systems

*2Fast mode is compatible with zoom 8-1000x and scanning modes X-Y and X-T. It is not compatible with Rotation, Free line, CROP, ROI, Spectral imaging, Stimulation and FLIM.


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