Bring imaging to life: confocal imaging advanced to levels previously unseen.
The A1+ confocal laser microscope system is Nikon's powerful fully-automated confocal imaging system, capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity. Ideal for facilities with a broad range of users, the A1+ has been designed with groundbreaking new optical and electronic technology innovations to provide unprecedented system quality and flexibility. New features such as Nikon's GaAsP multi-detector unit enables brighter, even higher resolution images than ever before.
ER: Enhance Your Confocal Resolution
The A1 ER enhanced resolution Module combines powerful GPU-based processing and specialized deconvolution algorithms to dramatically enhance the spatial resolution of your A1+ or A1R+ confocal microscope with minimal processing time.
The A1 ER Module provides high quality PSF models for Nikon’s high performance objective lenses, taking the guesswork out of deconvolution analysis. Other features include automatic or manual mode for iteration selection, enhanced spherical aberration correction, and robust algorithms for noise estimation and removal.
With A1 ER your theoretical resolution is your practical resolution.
Galvano Scanner Enables High-resolution Imaging
The A1+ utilizes a galvano scanner which enables high-resolution imaging of up to 4096 x 4096 pixels. In addition, with the newly developed scanner driving and sampling systems, plus image correction technology, high-speed acquisition of 10 fps (512 x 512 pixels) is also possible.
GaAsP Multi Detector Unit
A GaAsP PMT has much higher sensitivity than a normal PMT, thus acquisition of brighter signals with minimal background noise is possible with a GaAsP PMT, even with weak fluorescence, which, until now, has been difficult to detect.
When using resonant scanners, the GaAsP PMT enables low-noise, high-speed imaging.
Increased Light Detection Efficiency
incidence angle method
Reflection-transmission characteristics have high polarization dependence
Reflection-transmission characteristics have lower polarization dependence
By employing the hexagonal pinhole, higher brightness equivalent to that of a circular pinhole is achieved.
64% of the area of the circle
30% more light||
83% of the area of the circle
Nikon's original dual integration signal processing technology (DISP) has been implemented in the image processing circuitry to improve electrical efficiency, resulting in an extremely high S/N ratio.
Enhanced Spectral Imaging
Accurate, High-speed Unmixing
Accurate spectral unmixing provides maximum performance in the separation of closely overlapping fluorescence spectra and the elimination of autofluorescence. Superior algorithms and high-speed data processing enable real time unmixing during image acquisition.
Filter-less intensity adjustment is possible by selecting desired spectral ranges from 32 channels that match the spectrum of the fluorescence probe in use and combining them to perform the filtering function.
Increased flexibility and ease of use
NIS-Elements C control software enables integrated control of the confocal imaging system, microscope and peripheral devices with a simple and intuitive interface. Diverse reliable analysis functions are also available.