Localization-based super-resolution technique.
Nikon's N-STORM super-resolution system improves resolution by ~10 times over conventional imaging systems in that it does not observe all the fluorescently labelled molecules on the sample at the same time, but activates only a low percentage at any given time. At each time point/frame, Gaussian fitting enables precise localization of the centroid of each activated molecule image. By repeating this process and acquiring multiple frames, thousands of molecules in the field of view can be localized with nanometer accuracy. Combining these localizations results in the final super-resolution image.
STORM reveals nanoscale, molecular level details in biological samples. STORM techniques are being used for a variety of different fields of study including cell biology, microbiology, and neurobiology. Because of the need for successive image capture, STORM is most suited to the study of fixed specimens.
The Nikon STORM system (N-STORM), available through a license agreement with Harvard University, is based on the Eclipse Ti research level inverted microscope. The system provides multi-spectral 2-dimensional and 3-dimensional nanoscale imaging at the molecular scale. N-STORM acquisition and image reconstruction is carried out with Nikon's universal software platform, NIS-Elements.