The Nikon Imaging Centre at Fondazione Istituto Italiano di Tecnologia (NIC@IIT) is a core facility for light microscopy developed in partnership between Fondazione Istituto Italiano di Tecnologia and Nikon Instruments Italy. The NIC@IIT is to provide to a wide community of scientists and professionals throughout Italy, Europe and the Rest of the World, with the support of Nikon Instruments Italy, a large number of up-to-date imaging methodologies to monitor the living cell activity at high spatial and temporal rate. The main expertise of the NIC@IIT is related to Super resolution and multiphoton microscopy, and it is developed in the unique multidisciplinary environment of IIT. In the era of incredible advances in optical microscopy we can state that a new paradigm was born.
The mission of NIC@IIT is to:
The NIC@IIT is supported by the Chamber of Commerce of Genoa and by a selected number of partners.
Using high frequency Structured Illumination, the Nikon N-SIM can achieve image resolution of 85nm, which was previously considered impossible with optical microscopes. Temporal resolution of 0.6 sec/ frame enables super-resolution time-lapse imaging of dynamic live cell events.
The Eclipse Ti-E with Perfect Focus is Nikon's market leading inverted microscope system offering improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. Perfect Focus is Nikon's exclusive focus correction system that combats axial focus fluctuations in real time during long-term imaging investigations.
Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, Nikon's A1R is a powerful tool for the imaging and visualization of intracellular dynamics and interaction. The system comes equipped with a unique hybrid scan head incorporating both an ultra high-speed resonant scanner and a high-resolution galvano scanner, enabling simultaneous bleaching/ photoactivation and acquisition.
N-STORM is a super-resolution microscope system that combines “STochastic Optical Reconstruction Microscopy” technology (licensed from Harvard University) and Nikon's Eclipse Ti research inverted microscope. The N-STORM super-resolution microscope provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes.
Nikon’s A1R MP+ multiphoton confocal microscope is a unique multiphoton imaging system featuring a fast, high resolution galvanometer scanner and an ultra-high speed resonant scanner that is capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.
The Eclipse Ti-E inverted microscope with a Yokogawa Head is ideal for high speed imaging of living cells expressing fluorescent proteins or stained with membrane-permeant synthetic dyes. Photobleaching and phototoxicity are reduced with spinning disk microscopy. Samples can range from something as small as yeast through to a large specimen such as a Drosophila embryo.