Nikon (r)

Nikon Instruments Inc. | Americas

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University of California, San Francisco

Mission

The Nikon Imaging Center at UCSF/QB3 is a core facility for light microscopy developed in partnership with Nikon Instruments Inc. and Technical Instruments to:
Stimulate innovation in biological research by providing investigators access to cutting edge microscopy resources with a particular emphasis on developing novel imaging solutions to systems biology challenges.

  • Promote cross-discipline collaborations by providing an exciting intellectual commons centered on light microscopy.
  • Provide courses, individual instruction, and consulting on advanced light microscopy techniques.
  • Promote collaborations with Biopharmaceutical companies.

Systems Available

N-SIM on a Ti-E Inverted Microscope

Using high frequency Structured Illumination, the Nikon N-SIM can achieve image resolution of 85nm, which was previously considered impossible with optical microscopes. Temporal resolution of 0.6 sec/ frame enables super-resolution time-lapse imaging of dynamic live cell events.


Ti-E PFS Inverted Microscope System

The Eclipse Ti-E with Perfect Focus is Nikon's market leading inverted microscope system offering improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. Perfect Focus is Nikon's exclusive focus correction system that combats axial focus fluctuations in real time during long-term imaging investigations.


A1R Confocal with Ti-E Inverted Microscope

Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, Nikon's A1R is a powerful tool for the imaging and visualization of intracellular dynamics and interaction. The system comes equipped with a unique hybrid scan head incorporating both an ultra high-speed resonant scanner and a high-resolution galvano scanner, enabling simultaneous bleaching/ photoactivation and acquisition.

 


Nikon AZ-100

The AZ-100 Multizoom is a covers an extremely wide range of magnifications, from 5x to 400x, effectively combining the advantages provided by stereo zoom microscopes and compound microscopes. Thanks to a smooth zooming mechanism and a unique triple nosepiece, the AZ100 can continuously switch magnifications, extending from macro to micro observation of the same specimen.


Eclipse Ti-E TIRF / N-STORM Super-Resolution

The Eclipse Ti-E with Perfect Focus is Nikon's market leading inverted microscope system offering improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. Perfect Focus is Nikon's exclusive focus correction system that combats axial focus fluctuations in real time during long-term imaging investigations.

This total internal reflection fluorescence system is designed for high sensitivity imaging 100nm adjacent to the coverslip. This optical system eliminates background fluorescence by restricting the depth of the excitation region, increasing the spatial resolution in the optical plane of interest.

This microscope is also set up for super-resolution imaging using stochastic switching techniques such as (d)STORM, PALM and GSDIM. In this mode it can acquire images with a resolution as high as 20 nm in XY and 50 nm in Z.

 


Spinning Disk Confocal System

The Eclipse Ti-E with a Yokagawa CSU-X1 spinning disk confocal with Spectral Applied Research Aurora Borealis modification provides an ultra-fast and sensitive confocal microscope system ideal for live imaging.


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