The Nikon Imaging Center at Northwestern University Feinberg School of Medicine (NIC@NU-FSM) is an integral component of the Northwestern University Cell Imaging Facility, supported by Nikon, the Feinberg School of Medicine, Department of Cell & Molecular Biology and the Robert H. Lurie Comprehensive Cancer Center.
Established in 2008, the NIC@NU-FSM carries an important mission to:
Using high frequency Structured Illumination, the Nikon N-SIM can achieve image resolution of 85nm, which was previously considered impossible with optical microscopes. Temporal resolution of 0.6 sec/ frame enables super-resolution time-lapse imaging of dynamic live cell events.
Built on a reputation of incredible stability coupled with superior optical technologies, the C2 includes four channel confocal fluorescence imaging, and vastly expanded spectral capabilities with the ability to capture and unmix data acquired at any channel resolution across the entire detector bandwidth. The system is fully controlled by NIS-Elements imaging software.
The Biostation is an all-in-one, incubator microscope unit designed specifically for performing very long-term, multi-spot live cell imaging, The Biostation software can perform live cell imaging on multiple, stitchable spots on the sample, greatly increasing the experimental throughput as well as the field of view without losing resolution. The system can image red and green fluorophores in combination with phase contrast.
Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, Nikon's A1R is a powerful tool for the imaging and visualization of intracellular dynamics and interaction. The system comes equipped with a unique hybrid scan head incorporating both an ultra high-speed resonant scanner and a high-resolution galvano scanner, enabling simultaneous bleaching/ photoactivation and acquisition.
Nikon’s A1R MP+ multiphoton confocal microscope is a unique multiphoton imaging system featuring a fast, high resolution galvanometer scanner and an ultra-high speed resonant scanner that is capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.