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Heinrich Pette Institute - Hamburg

MISSION

The NIKON Center of Excellence is a cooperation project between the Heinrich Pette Institute, Leibniz Institute for Experimental Virology and the Nikon GmbH business unit microscopy solutions. The aim of this cooperation is the support of the researchers with the latest light microscopy technology as well as the development and establishment of new tailor-made preparation and transfer methods for correlative and integrative imaging.

Based on this collaboration a stochastic optical reconstruction microscopy N-STORM system is available to the technology platform "Microscopy and Image Analysis". This new microscopy technique enables an increase in optical resolution by a factor of ten compared to conventional fluorescence microscopy. For this new technology complementary correlative light-and electron microscopy techniques are being developed at the HPI. In addition to the N-STORM, the Nikon GmbH provides various other equipment and know-how to the HPI scientists and guests.

Systems Available

N-STORM Localization-based Super-resolution Microscope

N-STORM is a super-resolution microscope system that combines “STochastic Optical Reconstruction Microscopy” technology and Nikon's Eclipse Ti research inverted microscope. The N-STORM super-resolution microscope provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes and enables molecular level understanding.


Ti-E PFS Inverted Microscope System

The Eclipse Ti-E with Perfect Focus is Nikon's market leading inverted microscope system offering improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging. Perfect Focus is Nikon's exclusive focus correction system that combats axial focus fluctuations in real time during long-term imaging investigations.


C2+ Confocal Microscope with Ti-E Inverted Microscope

Built on a reputation of incredible stability coupled with superior optical technologies, the C2 includes four channel confocal fluorescence imaging, and vastly expanded spectral capabilities with the ability to capture and unmix data acquired at any channel resolution across the entire detector bandwidth. The system is fully controlled by NIS-Elements imaging software.


Spinning Disk Confocal System

The Eclipse Ti-E with a Yokagawa CSU-X1 spinning disk confocal with Spectral Applied Research Aurora Borealis modification provides an ultra-fast and sensitive confocal microscope system ideal for live imaging.


Nikon Biostation Incubator Microscope

The Biostation is an all-in-one, incubator microscope unit designed specifically for performing very long-term, multi-spot live cell imaging, The Biostation software can perform live cell imaging on multiple, stitchable spots on the sample, greatly increasing the experimental throughput as well as the field of view without losing resolution. The system can image red and green fluorophores in combination with phase contrast.


Nikon AZ-100

The AZ-100 Multizoom is a covers an extremely wide range of magnifications, from 5x to 400x, effectively combining the advantages provided by stereo zoom microscopes and compound microscopes. Thanks to a smooth zooming mechanism and a unique triple nosepiece, the AZ100 can continuously switch magnifications, extending from macro to micro observation of the same specimen.


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