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Florida Atlantic University

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Center of Excellence

Florida Atlantic University

The FAU Nikon Center of Excellence, a partnership with the FAU Brain institute, seeks to provide scientists and trainees across the university’s six campuses with access to high-performance, cellular imaging tools to advance the study of fundamental biological mechanisms and their contributions to human disorders. 

Through our Nikon partnership, the FAU community and its community partners gain access to Nikon’s technical expertise and advanced training support on existing imaging platforms as well as opportunities to explore leading-edge Nikon imaging technologies at the earliest possible stages of development. Installations on the Jupiter, FL campus include multiple confocal platforms with enhanced resolution capability, automated X-Y-Z image capture, and TIRF technology, affording high resolution analysis of single molecules in cellular sub-compartments and large field image capture and reconstruction.

A complete list of available systems can be found on the FAU website link below.

Contact

CofE Director

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CofE Manager

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Address

Florida Atlantic University
5353 Parkside Dr.
Jupiter, FL 33458


Systems Available

TIRF/A1R HD Resonant Scanning Confocal System

This A1R HD resonant scanning confocal system is ready for the most demanding live cell and tissue imaging applications, featuring stage-top incubation, fast Z piezo stage, and spectral detector for advanced multicolor imaging applications.

Components

  • ECLIPSE Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1R HD high-definition resonant scanning confocal system

  • A1-DUS 32-channel spectral detector
  • Motorized TIRF Illuminator Unit
  • 6-line laser unit (408 nm, 455 nm, 488 nm, 514 nm, 561 nm, 640 nm)
  • Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
  • DU4G GaAsP 4-channel confocal detector
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

A1R MP Resonant Scanning Multiphoton System

This fast multiphoton system is based on an A1R MP resonant scanning multiphoton on an ECLIPSE FN1 upright microscope and is ideal for intravital imaging applications.

Components

  • ECLIPSE FN1 upright microscope

  • A1R MP resonant scanning multiphoton system
  • Coherent Chameleon Ultra II Ti:S laser unit (tunable 680-1080 nm)
  • Nosepiece-mounted Z-axis objective piezo
  • Episcopic non-descanned detectors (NDDs)
  • Prior ZDeck Platform System
  • NIS-Elements software

N-SIM E/A1R HD Super-Resolution & Confocal System

This correlative confocal and super-resolution system features both N-SIM E structured illumination and A1R HD resonant scanning confocal, giving users the flexibility to prioritize speed or resolution.

Components

  • ECLIPSE Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)

  • N-SIM E super-resolution structured illumination microscopy system

  • A1R HD high-definition resonant scanning confocal system

  • DU4G GaAsP 4-channel confocal detector
  • LUN-4 4-line laser unit (405 nm, 488 nm, 561 nm, 640 nm) for confocal
  • LUN-3 3-line laser unit (488 nm, 561 nm, 640 nm)
  • Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

A1R HD25 Resonant Scanning Confocal System

This A1R HD25 resonant-scanning confocal system provides fast automated imaging on our motorized ECLIPSE Ni-E upright microscope.

Components

  • ECLIPSE Ni-E upright microscope

  • A1R HD25 high-definition resonant scanning confocal system

  • DU4G GaAsP 4-channel confocal detector
  • LUN-4 4-line laser unit (405 nm, 488 nm, 561 nm, 640 nm)

  • Lumencor SOLA light engine
  • NIS-Elements software

Upright A1 Confocal System

This A1 point-scanning confocal system is configured on an ECLIPSE Ni-E upright microscope and is a great option for applications requiring confocal-level optical sectioning.

Components

  • ECLIPSE Ni-E upright microscope

  • DU4 4-channel confocal detector
  • LUN-4 4-line laser unit (405 nm, 488 nm, 561 nm, 640 nm)

  • Lumencor SOLA light engine
  • NIS-Elements software

  • NIS-Elements Offline Analysis Workstation

Upright A1R Confocal System

This A1R resonant-scanning confocal system on our ECLIPSE FN1 microscope is perfect for in vivo high-speed imaging experiments and correlative electrophysiology work.

Components

  • ECLIPSE FN1 upright microscope

  • DU4G GaAsP 4-channel confocal detector
  • LUN-4 4-line laser unit (408nm, 488nm, 561nm, 640nm)

  • Lumencor SOLA light engine
  • NIS-Elements software