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Cincinnati Children’s Hospital Medical Center

Mission

Nikon Instruments USA has partnered with Cincinnati Children’s Hospital Medical Center, to open a Nikon Center of Excellence for advanced biological imaging. The center will combine Nikon’s technical proficiency with the scientific expertise of the researchers at Cincinnati Children’s Research Foundation to further new developments in biomedical sciences that will ultimately improve child health.

Area of Expertise

This partnership provides CCRF researchers access to state-of-the art imaging equipment and Nikon technical expertise. In return, Nikon gains valuable feedback and insight from talented scientists using advanced research techniques in the diverse areas of research strength found at the CCRF. Our expertise includes phenotypic imaging and analysis of complex biological systems including developmental biology, neurobiology, stem cell biology and cancer biology. We also have a keen focus on developing and adapting new imaging techniques that can be applied to our researcher's areas of expertise.

Systems Available

A1R GaAsP N-STORM Super-Resolution System on an Ti-E Inverted Microscope with LUN-V Laser Launch

The A1R with GaAsP Multi Detector Unit is capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity. Also included is N-STORM, a powerful super-resolution microscope system that combines “STochastic Optical Reconstruction Microscopy” technology and Nikon's Eclipse Ti research inverted microscope. This system is equipped with an LUN-V laser launch which provides multiple output fibers and flexible laser choices enabling multiple imaging modalities to share the same laser combiner.

  • Inverted, motorized Eclipse Ti inverted microscope on an A1R confocal
  • High-power 405, 445, 488, 514, 561, and 647nm solid state diode lasers on Nikon's monolithic laser combiner
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • Piezo-Z stage insert for high-speed Z-stack acquisition (100μm/s)
  • Dual scanner: resonant and galvanometric
  • Two high-quantum efficiency, low-noise Hamamatsu photomultiplier tubes, a transmitted PMT for transmitted light, and two very high-QE Gallium-Arsenide-Phosphide PMTs for overall 400-820nm detection
  • Nikon Perfect Focus System for maintaining focal plane during time-lapse experiments
  • Available objectives:
    • 4X Plan Apo
    • 10X Plan Apo λ
    • 20X Plan Apo VC DIC
    • 40X Apochromatic λS DIC-Water Immersion
    • 60X Plan Apo IR DIC- Water Immersion
    • 100X Plan Apo TIRF DIC- Oil Immersion
    • STORM Super-resolution (lateral resolution down to 15nm)
    • TIRF, FRAP

A1R GaAsP Confocal with Ti-E Inverted Microscope

Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, Nikon's A1R is a powerful tool for the imaging and visualization of intracellular dynamics and interaction. Also equipped with GaAsP detectors which offer much higher sensitivity than normal PMTs, enabling fast, low-light confocal imaging.

  • Inverted, motorized Eclipse Ti inverted microscope on an A1R confocal
  • 405nm, 561nm, and 640nm diode lasers, and a multi-line Argon (457nm, 471nm, 488nm, 514nm)
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • Piezo-Z stage insert for high-speed Z-stack acquisition (100μm/s)
  • Dual scanner: resonant and galvanometric
    • Resonant: high speed-30 fps at 512 x 512. Up to 400 fps at 32 x 512
    • Galvanometric: high resolution- up to 4K scan area
  • Two high-quantum efficiency, low-noise Hamamatsu photomultiplier tubes, a transmitted PMT for transmitted light, and two very high-QE Gallium-Arsenide-Phosphide PMTs for overall 400-820nm detection
  • Nikon Perfect Focus System for maintaining focal plane during time-lapse experiments
  • Equipped with Andor 888 Ultra EMCCD camera and dark box for luminescence imaging
  • Available objectives:
    • 4X Plan Apo
    • 10X Plan Apo λ
    • 20X Plan Apo VC DIC
    • 40X Apochromatic λS DIC-Water Immersion
    • 60X Plan Apo IR DIC- Water Immersion
    • 100X Plan Apo DIC- Oil Immersion

A1 GaAsP LUN-A Inverted Confocal Microscope

  • Inverted, motorized Eclipse Ti inverted microscope on an A1 confocal
  • 405nm, 488nm, 561nm, and 640nm diode lasers in Nikon's small-footprint monolithic laser combiner
  • Fully encoded scanning XY motorized stage for multipoint and tile scanning
  • Two high-quantum efficiency, low-noise Hamamatsu photomultiplier tubes and two very high-QE Gallium-Arsenide-Phosphide PMTs for overall 400-820nm detection
  • Available objectives:
    • 4X Plan Apo
    • 10X Plan Apo λ
    • 20X Plan Apo VC DIC
    • 40X Plan Apo DIC
    • 60X Plan Apo IR DIC- Water Immersion
    • 100X Plan Apo DIC- Oil Immersion

A1R GaAsP LUN-V Inverted Confocal Microscope

  • Inverted, motorized Eclipse Ti inverted microscope on an A1 confocal
  • 405, 445, 488, 514, 561, and 647nm solid state diode lasers on Nikon's monolithic laser combiner
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • Galvanometric: high resolution- up to 4K scan area
  • Two high-quantum efficiency, low-noise Hamamatsu photomultiplier tubes, a transmitted PMT for transmitted light, and two very high-QE Gallium-Arsenide-Phosphide PMTs for overall 400-820nm detection
  • Nikon Perfect Focus System for maintaining focal plane during time-lapse experiments
  • Available objectives:
    • 4X Plan Apo
    • 10X Plan Apo λ
    • 20X Plan Apo VC DIC
    • 40X Apochromatic λS DIC-Water Immersion
    • 60X Plan Apo IR DIC- Water Immersion
    • 100X Plan Apo DIC- Oil Immersion

A1Rsi Confocal on a Ti-E Inverted Microscope

Nikon’s powerful fully-automated confocal imaging system with a spectral detector for high-speed spectral data acquisition and spectral unmixing.

  • Inverted, motorized Eclipse Ti inverted microscope on an A1R confocal
  • 405nm, 561nm, and 647nm diode lasers, and a multi-line Argon (457nm, 471nm, 488nm, 514nm)
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • Piezo-Z stage insert for high-speed Z-stack acquisition (100μm/s)
  • Dual scanner: resonant and galvanometric
    • Resonant: high speed-30 fps at 512 x 512. Up to 400 fps at 32 x 512
    • Galvanometric: high resolution- up to 4K scan area
    • Dual scanner: resonant and galvanometric
  • Four high-quantum efficiency, low-noise Hamamatsu photomultiplier tubes and a transmitted PMT for transmitted light and 400-820nm detection
  • 32 Channel multi-anode PMT spectral detector
  • Nikon Perfect Focus System for maintaining focal plane during time-lapse experiments
  • Available objectives:
    • 4X Plan Apo
    • 10X Plan Apo λ
    • 20X Plan Apo VC DIC
    • 40X Apochromatic λS DIC-Water Immersion
    • 60X Plan Apo IR DIC- Water Immersion
    • 100X Plan Apo DIC- Oil Immersion

A1R Multiphoton with FN1 Upright Microscope

Nikon's A1R MP multiphoton microscope enables high speed, deep tissue imaging in live organisms with unprecedented sensitivity and clarity. The system features an ultra-high speed resonant scanner capable of 420 fps imaging, a galvanometric scanner for high-resolution imaging, high-sensitivity GaAsP Multi Detector in both episcopic and diascopic imaging paths to optimize signal detection and high N.A. objective lenses designed for high-resolution, deep tissue imaging.

  • Upright FN 1 microscope on an A1R confocal
  • 405nm, 488nm, 561nm, and 640nm diode lasers
  • Coherent Chameleon II TiSapphire IR laser, tuneable from 700-1000nm
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • Piezo-Z nosepiece for high-speed Z-stack acquisition (100μm/s)
  • Dual scanner: resonant and galvanometric
  • Four high-quantum efficiency, low-noise Hamamatsu photomultiplier tubes, a transmitted PMT for transmitted light for 400-820nm detection
  • Four high-quantum efficiency GaAsP non-descanned detectors for multiphoton imaging
  • Available objectives:
    • 16X Apo 0.8 NA LWD Water Immersion
    • 25X Apo 1.1 NA LWD Water Immersion

A1 Confocal with Ti-E Inverted Microscope

The A1 confocal laser microscope system is Nikon's powerful fully-automated confocal imaging system, capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity.

  • Inverted, motorized Eclipse Ti inverted microscope on an A1R confocal
  • 405nm, 561nm, and 640nm diode lasers, and a single-line 488nm Argon
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • Galvanometric scanner for up to 4K scan area
  • Four high-quantum efficiency, low-noise Hamamatsu photomultiplier tubes and a transmitted PMT for transmitted light and 400-820nm detection
  • Available objectives:
    • 4X Plan Fluor
    • 10X Plan Apo
    • 20X Plan Apo
    • 40X Apochromatic λS DIC-Water Immersion
    • 60X Plan Apo- Water Immersion
    • 100X Plan Apo DIC- Oil Immersion

Eclipse Ti-E Inverted Microscope

This system is equipped with high-speed triggered acquisition, multi-band pass filter cubes, Prior LED transmitted illumination (for high-speed switching of DIC and fluorescence).

  • Inverted, motorized Eclipse Ti-E microscope
  • Nikon DS-Fi2 5 megapixel, 8-bit color CCD camera
  • Andor Xyla 4.2 megapixel, 16-bit sCMOS monochromatic camera
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • High-speed, triggerable Lumencor SpectraX LED light engine for high-speed multichannel fluorescence image acquisition
  • Available objectives:
    • 4X Plan Apo
    • 10X Plan Apo
    • 20X Plan Apo
    • 40X Plan Apo
    • 10X CFI Achro Phase
    • 20X CFI Achro Phase

Eclipse Ni-E Upright Microscope

The Eclipse Ni-E is a versatile upright microscope ideal for high-speed, eli-fluorescence imaging. The proprietary "stratum structure" design and expandable infinity space enables the addition of multiple turrets, detectors, laser inputs and other accessories. 

  • Eclipse Ni-E upright with Andor Zyla 4.2 sCMOS Camera
  • Prior Motorized XY Stage
  • Nikon DS-Fi2 5 megapixel, 8-bit color CCD camera
  • Andor Zyla 4.2 megapixel, 16-bit sCMOS monoschromatic camera
  • Lumencor Sola LED Illumination
  • Available objectives: 
    • 2X Plan Apo
    • 10X Plan Apo 
    • 20X Plan Apo
    • 40X Plan Apo
    • 60X Plan Apo- Oil Immersion

Eclipse 90i Upright Microscope

The Eclipse 90i delivers a completely integrated, totally optimized digital microscopy system engineered to master the most demanding applications. The microscope includes a motorized digital head, proprietary "fly-eye" lens array, and is configured for multi-point time-lapse and tile scanning (widefield fluorescence).

  • Upright, motorized Eclipse 90i microscope
  • Nikon DS-Fi2 5 megapixel, 8-bit color CCD camera
  • Photometrics CoolSnap HQ2 14-bit CCD monochromatic camera
  • Fully encoded scanning XY motorized stage for multipoint time lapse and tile scanning
  • Lumencor Sola LED light engine
  • Available objectives:
    • 4X Plan Fluor
    • 10X Plan Apo
    • 20X Plan Apo
    • 40X Plan Apo
    • 60X Plan Apo- Oil Immersion
    • 100X Plan Apo DIC- Oil Immersion

Specialty Optics Shared Between Systems

  • Two 20X CFI Apo LWD WI NA 0.95 Lambda S Objectives
  • Two Multi-immersion 20X Objectives
  • Two VC plan apo 60X oil Objectives
  • Super Fluor 20X 
  • Super Fluor 10X

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