Nikon (r)

Nikon Instruments Inc. | Americas

Skip to main content

Center for Gastrointestinal Biology of UFMG - Brazil

UFMG Campus – Belo Horizonte, Brazil.

The Nikon Center of Excellence at the Center for Gastrointestinal Biology (CGB) in Federal University of Minas Gerais (UFMG) is focused in providing imaging solutions to researchers that need to understand the mechanisms behind their projects imaging ions flux (i.e. calcium waves), immune cell migration and interaction and distribution and expression of several different molecules specially in their most native context: in vivo. For this, CGB has a very flexible imaging core that allows the imaging in vivo of single cells, organ slices and whole organs in a living mouse using multi-channel confocal intravital microscopy. Nikon partnership has enhanced our ability to adapt stock microscopes to become high performance and accessible platforms for in vivo imaging. It is important to mention that conventional slices techniques, including 3D rendering of immunohistochemistry slices and basic fluorescence experiments can also be done using the same microscopes at our CofE. We make all efforts to serve the whole UFMG and other Minas Brazilian Universities, adapting our microscopes to the different demands. The expertise, accessibility and advanced training provided by Nikon has allowed even very young students since the beginning of their graduating career to understand, learn and actually manipulate and use by themselves our Nikon confocal microscopes. Our imaging core has 2 confocals, and both that can be used for in vivo or ex vivo imaging. Using delicate surgical techniques and animal life support and care, we can image in high resolution, and even in three dimensions using automatic Z-stacks different cell behaviour in vivo during several hours in a living mouse. This produces amazing movies  that have enhanced our knowledge in different areas.

Systems Available

A1R Si Confocal Microscope on a Ti-E Inverted Microscope

The A1R confocal microscope is a high-quality imaging system with a high-resolution Galvano scanner and high-sensitive PMT’s , reaching a resolution of up to 4096 x 4096 pixels. By combining the resonant scanner (up to 420 frames per second) and the motorized Piezo Z stage, high-speed 3D scanning can be achieved for live imaging. Using the spectral imaging detector, up to 32 channels can be acquired simultaneously. The A1R Si is additionally equipped with Nikon’s resonant system, allowing high speed imaging acquisition.

Components

  • Nikon Ti-E Inverted Microscope
  • Fully Motorized Stage
  • Six Laser Lines: 408, 445, 488, 514, 561, 640
  • Four Detectors: blue, green, red, far red (High Sensitive PMT)
  • Spectral Detector (maximum 32 channels, 400-700nm range)
  • High-Resolution Galvano Scanner (up to 4096 x 4096 pixels)
  • High-Speed Resonant Scanner (up to 420 frames per second)
  • Perfect Focus System (dynamic focus system)
  • Epi Light
  • Light Source, with three LED lights (red, blue, green) for color images
  • Objectives: Plan Fluor 4x, Plan Apo λ 10x, Plan Apo 40x, Plan Apo VC 60x Oil
  • 64bit Imaging Workstation, Windows 7
  • NIS-Elements Imaging Software
  • 2 Monitors
  • Air Table (36”x48”)

GFP-expressing-E. coli spreading after injection within liver microvasculature


C2 Confocal Microscope on an Eclipse Ti Inverted Microscope

The Nikon C2 confocal microscope is an easy to operate imaging system, used for a wide range of imaging applications. In addition to the three standard detectors, this confocal can capture transmitted light.


Components

  • Nikon Eclipse Ti inverted Microscope
  • Four Laser Lines: 405, 488, 561
  • Three Standard Detectors (detection of three channels simultaneously: blue, green, red, and transmitted light)
  • Fluorescent Light Source
  • Objectives: Plan Apo λ 4x, Plan Fluor 10x, Plan Apo 60x Oil
  • 64bit Imaging Workstation, Windows 7
  • NIS-Elements Imaging Software
  • 2 monitors

GFP expressing-E. coli captured by liver macrophages that inhabit the lumen of the vessels.

3D reconstruction of liver vasculature and the extravascular population of dendritic cells.

Green neutrophils swarming to a necrotic area

4D generation of intravital movies. Green neutrophils rapidly swarm to a necrotic area.


Back to top