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Stem Cell Biology
the development of natural and synthetic tissues/cells for implantation into the body

Live mouse gut organoid. Fragments of gut tissue can be grown in vitro and images taken while they are alive to study cell motility. Image courtesy of Dr. Paul Appleton, College of Life Sciences, University of Dundee

The development of natural and synthetic tissues and/ or cells for implantation into the body to restore or establish normal function

Regenerative medicine involves the use of cell-derived therapeutics, stem cell therapies, and the use of advanced biomaterials for scaffolds in tissue engineering and transplants. Stem cell technologies are a key focus in regenerative medicine. By culturing and guiding differentiation of stem cells in the laboratory, the hope is to generate tissue-specific cells and tissues that can be introduced into the body to help repair organs or to express vital proteins. A pluripotent stem cell can remain undifferentiated during sub culture but is capable of differentiating into virtually any type of tissue induced by specific cell culture conditions. Stem cell culture, therefore, requires the carefully controlled conditions provided by the Nikon IM-Q and CT BioStation time-lapse incubator imaging systems, which promote consistent and healthy cell populations and enable cell growth in culture to be easily monitored. Identification and characterization of stem cells in culture and in tissue can be carried out with Nikon’s suite of microscopes from the Eclipse TS100 tissue culture microscope to high zoom stereomicroscopes, confocal and multiphoton microscopes, and super-resolution microscopes that enable imaging with molecular resolution.

Imaging Challenges

How can I prevent focal drift?

Changes in temperature and mechanical vibrations can occur during long-term imaging experiments.  These problems in turn induce focal drift. Nikon’s Perfect Focus 3 System offers continuous focus correction – keeping your sample in focus over long periods of time ensuring crisp clean images and movies.

SOLUTION
Perfect Focus

How can I keep my cells alive for long periods of time in live cell imaging?

Light exposure (phototoxicity), temperature and time can affect the longevity of cells. Nikon’s BioStation CT and IM-Q time-lapse imaging incubator systems provide environmentally controlled incubation with an integrated and automated microscope for monitoring cells as they divide and differentiate in optimal culture conditions.

SOLUTION
BioStation IM-Q

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