the prevention, diagnosis and management of reproductive problems
The prevention, diagnosis and management of reproductive problems
A key technology in this area is in vitro fertilisation (IVF). IVF was originally used in women who had difficulty conceiving. Today it is relevant to almost all fertility problems in both men and women when combined with intracytoplasmic sperm injection (ICSI). Several important procedures in IVF involve sperm evaluation and sperm and egg micromanipulation and pre-implantation diagnosis under microscopic observation. Sperm selection can be based on normal sperm head morphology using a high magnification imaging technique called intracytoplasmic morphology selected sperm injection (IMSI), an imaging technique that requires >6000x magnification. Microscopes used for IVF must be exceptionally stable and vibration free with space to accommodate micromanipulation equipment. Nikon’s Eclipse Ti provides high quality optics, a stable vibration-free body and additional magnification lenses. Combined with Nikon’s long working distance, high N.A. DIC and NAMC objectives, the Ti is an ideal platform for performing ICSI and IMSI. Various temperature regulation accessories are available to maintain the correct temperature for these critical IVF procedures. Nikon’s large zoom-range stereomicroscopes and high N.A. objectives are ideal for examining oocytes and embryos. Nikon’s incubator-imaging systems, the BioStation CT and IM-Q provide a powerful means to monitor embryo development with minimal perturbation to its culture environment. Nikon’s clinical upright microscope, the Eclipse Ci, provides a robust imaging platform for routine histology and pathology work. Combined with Nikon’s Digital Sight series cameras and DS-L3 imaging system, telepathology is possible. Telepathology/cytology technologies greatly assist in real-time assessment by pathologists in remote locations, in training, and archiving specimens.
How can I keep my cells alive for long periods of time in live cell imaging?
Light exposure (phototoxicity), temperature and time can affect the longevity of cells. Nikon’s BioStation CT and IM-Q time-lapse imaging incubator systems provide environmentally controlled incubation with an integrated and automated microscope for monitoring cells as they divide and differentiate in optimal culture conditions.