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Nikon Instruments Introduces New Silicone Immersion Objective Lens for Super-Resolution and Multi-Modal Imaging

May 22, 2017

CFI SR HP Plan Apochromat Lambda S 100XC Sil objective and silicone immersion medium enable superresolution imaging of thick specimens and offers correlative imaging capabilities

CFI SR HP Plan Apochromat Lambda S 100XC Sil

Nikon Instruments Inc. today unveiled a new silicone immersion objective lens, CFI SR HP Plan Apochromat Lambda S 100XC Sil, for super-resolution and multi-modal imaging. The new silicone immersion objective lens from Nikon provides high numerical aperture and long working distance to enable super-resolution imaging of thick samples as well as correlative imaging with conventional applications such as confocal. Nikon Instruments will unveil the CFI SR HP Plan Apochromat Lambda S 100XC Sil at the 17th International European Light Microscopy Initiative Meeting, May 23-26 in Dubrovnik, Croatia.

“As super-resolution imaging techniques such as SIM and STORM grow in popularity, there are increasing demands to adapt these applications to thicker samples and to correlate data acquired with different super-resolution and conventional techniques,” said Steve Ross, Ph.D., General Manager, Products and Marketing, Nikon Instruments Inc. “We’re pleased to introduce an objective lens to meet those needs.”

CFI SR HP Plan Apochromat Lambda S 100XC Sil Features

  • Enables High resolution Imaging of Thick Specimens - The CFI SR HP Plan Apochromat Lambda S 100XC Sil objective is designed for use with Nikon’s new silicone oil to provide high resolution imaging of thick samples.  Nikon’s newly developed silicone oil closely matches the refractive index of the specimen to reduce spherical aberration when imaging through thick samples, resulting in bright and clear images.  It also achieves excellent viscosity to maintain the immersion liquid during long-term observation and time-lapse imaging.
  • Ideal for super-resolution microscopy "N-SIM" "N-STORM" - The new silicone immersion objective is compatible with both structured illumination (N-SIM) and single-molecule localization (N-STORM) based super-resolution techniques. It is highly corrected for axial chromatic aberration and supports high laser power transmission. Combined with its high N.A. and long working distance, the objective enables 3D, multi-color super-resolution imaging of thick samples.
  • Achieves accurate chromatic aberration reduction and high transmission rates across a broad spectrum, from UV to near IR - Chromatic aberration is precision-corrected over a wide wavelength range from visible to ultraviolet. Nikon’s highly acclaimed, proprietary, anti-reflective Nano Crystal Coat is utilized to achieve high transmission from UV to near IR wavelengths.
  • Improves operability for multi-imaging modalities - The new silicone immersion objective is compatible with confocal systems to enable correlative imaging between confocal and super-resolution techniques.  It also eliminates the need to switch objective lenses between N-SIM and N-STORM, providing a seamless workflow between different super-resolution techniques.

Specifications

Model name CFI SR HP Plan Apochromat Lambda S 100XC Sil
Magnification 100X
Numerical aperture 1.35
Working distance 0.3 mm
Parfocal distance 60 mm
Correction ring Equipped
Usable cover glass thickness 0.15 - 0.19 mm
Usable temperature 23 to 37°C /73.4 to 98.6° F

The CFI SR HP Plan Apochromat Lambda S 100XC Sil objective is now available. Visit https://www.nikoninstruments.com/cfi-sr-hp-apo

ABOUT NIKON INSTRUMENTS INC.

Nikon Instruments Inc. is a world leader in the development and manufacture of optical and digital imaging technology for biomedical applications. Now celebrating its 100th year, Nikon provides complete optical systems that offer optimal versatility, performance and productivity. Cutting-edge instruments include microscopes, digital imaging products and software. Nikon Instruments is one of the microscopy and digital imaging arms of Nikon Inc., the world leader in digital imaging, precision optics and photo imaging technology. For more information, visit www.nikoninstruments.com. Product-related inquiries may be directed to Nikon Instruments at 800-52-NIKON.

Press Contact:

Trisha Bruynell
Peppercomm
tbruynell[at]peppercomm.com


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