| Scan head:
| Compact scan head features fiber optic connections for laser input and both spectral and standard detector units, user interchangeable scan head dichroic mirrors, and 4 PC controlled confocal pinholes in a rotating drum.
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| Acquisition Parameters:
| Simultaneous spectral acquisition of up to 32 channels in a single scan within a 350nm spectral bandwidth from 400-750nm. The 32 channel spectrum can be acquired anywhere within the bandwidth at user selected channel widths of 2.5nm, 5nm, and 10nm. Binning and masking of channels is provided. Maximum frame size in spectral mode is 1024 x 1024 pixels acquired in 2 sec. Faster acquisition is possible at reduced image size in band scan. Spectral acquisition of 2D X/Y, 3D X/Y/Z, 3D X/Y/T, 4D X/Y/Z/T images and data is supported. Continuous scan zoom and full navigation functions available in spectral mode.
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| Spectral Detector Unit:
| The fiber coupled spectral detector unit contains three diffraction gratings supporting 2.5nm, 5nm, and 10nm channel widths mounted on a rotating drum. Polarization rotating optics are included that rotate all emission photons to the highest efficiency s-plane polarization. The detector itself is a 32 element multi-anode PMT with a movable laser blocking plate.
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| Standard Detector Units:
| The fiber coupled 3 channel standard detector unit contains 3 extended red sensitivity side window PMT's. Emission filters and dichroic mirrors are mounted in standard Nikon EF-4 filter blocks and are easily user interchangeable. A motorized, fiber coupled, scanned transmitted light detector with a dedicated mini-PMT is also available.
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| Laser Unit:
| Three laser unit with acousto-optical modulator (AOM) control or direct electronic modulation of laser intensity. All beams are combined and launched through a single mode polarization maintaining optical fiber. A four laser unit is also available with direct modulation of diode lasers and acousto-optical tunable filter (AOTF) modulation of the other lasers.
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| Lasers:
| 488nm single line and 4-line multiline Argon ion lasers, 405nm, 440nm, and 638nm directly modulated diode lasers, 561nm diode pumped solid state (DPSS), and 488nm solid state.
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| Z-Control:
| 50nm step size with TE2000E and Nikon Eclipse 90i automated microscopes or accessory focus drive units capable of 50nm step increments.
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| Microscope compatibility:
| Nikon Eclipse Series 80i and 90i, E-600 and E-800 upright microscopes, FN1 and E600FN fixed stage physiology microscopes, and Nikon Eclipse Series TE-2000E, TE2000U, and TE-300 inverted microscopes.
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| Software:
| EZ-C1 acquisition and analysis software supporting standard and spectral confocal acquisition, spectral unmixing, spectral graphing and presentation, 3D rendering visualization and time series in both spectral and standard modes, and Visual basic Applications.
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| Optical system:
| Recommended objective lenses include Nikon CFI60 VC Plan Apo, Plan Apo, Plan Apo TIRF, Plan Fluor, S Fluor, and W Fluor series lenses.
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