The C1 Plus supports almost any imaging technique you use today, including simultaneous 3-channel fluorescence, 3-channel plus DIC, time-lapse acquisition, and spatial analysis.

We've fused our first-class optics and electronics technologies to bring you a confocal system that provides state of the art resolution, contrast, and fluorescence image brightness.

The laser unit holds up to 3 different lasers, allowing you to use a variety of fluorescent probes. Also, changing filters to match the fluorescent dyes you're using is simple and fast. So as your research needs change, so will your microscope. A four laser unit is also available with acousto-optical tunable filter (AOTF) modulation of all lasers. Laser choices include Argon ion, HeNe, Diode, and diode pumped solid state lasers (DPSS).

The C1 Plus has a number of scanning features to make image acquisition simpler and faster. These include:

• Bidirectional Scanning: To improve image scan speed.
• Scan Rotation: The image direction of long specimens, such as neurons, can be rotated without rotating the XY platform
• Variable Delay in Time Lapse: Allows the intervals in time-lapse recordings to be varied.

• Modular Components: Expansion and maintenance are easy.
• Pre-Calibrated Modules: No need for calibration during set-up.
• Compact Design: Does not fill up bench space. The C1 Plus features the world's smallest and lightest scan head.
• Intuitive Software: Most parameters can be modified with a single click of the mouse.
• Four Position Pinhole Turret: Computer controlled selection of pinhole aperture allows customized balance between resolution and optical thickness.

Fluorescence recovery after photo bleaching (FRAP) involves bleaching a targeted area of a cell with intense laser exposure and observing the recovery process. This is a useful tool for measuring the speed of molecular diffusion and movement. The C1 Plus can precisely target the laser exposure to the region of interest.

The Nikon laser TIRF system can be used as a stand alone imaging device, in combination with widefield fluorescence, and/or in combination with a confocal microscope like C1-Plus or C1si. TIRF (total internal reflection fluorescence) microscopy improves signal to noise by exciting only those molecules located within a thin evanescent field, typically within 100nm or less of the coverslip. This technique complements confocal microscopy. The Nikon laser TIRF system mounts on the Eclipse Ti in place of the standard fluorescence illuminator. It incorporates both TIRF and widefield fluorescence microscopy in a single device. Nikon has introduced two outstanding TIRF Plan Apochromat lenses with magnifications of 60x and 100x and NA = 1.49. These are the highest numerical aperture oil immersion objective lenses available anywhere.