Key Words: Nikon C1, 3-D
Definition:Extended depth of field is an image processing method to obtain images of 3-D objects and organisms. Depth of field refers to the distance between the nearest and furthest object in an image in acceptably sharp focus, i.e. the zone of sharpest focus in front of, around and behind the subject
TECHNOLOGY:
The hardware and supporting software packages enable the user to display a stack of images as a composite, effectively creating a 3-D image. This process of viewing composite images as one uncovers a vast amount of detail that may otherwise go unnoticed, and requires an image volume of at least two image planes.
In microscopy depth of field is very short and usually measured in units of microns. The term depth of focus, which refers to image space, is often used interchangeably with depth of field, which refers to object space. Depth of focus varies with numerical aperture and magnification of the objective, and under some conditions, high numerical aperture systems (usually with higher magnification power) have deeper focus depths than do those systems of low numerical aperture, even though the depth of field is less.
Formulas can be applied to calculate the exact depth of field.
APPLICATIONS:
Applications include the elucidation of previously unseen structures and determining the relationships between existing structures.
Extended depth of field can be used with the spectrum of microscopical techniques from standard bright field through to confocal. It is useful in making direct qualitative assessments about the structure and composition of image volumes.
Extended depth of field has both biological and industrial applications. Industrial uses can involve application in combination with metallurgical microscopes for analysis of such things as material stress, strain and fatigue. Whereas biological applications are broad and far reaching.
MICROSCOPE CONFIGURATION:
Nikon C1 (ICS)
SYSTEM SOLUTION:
Please consult your local Nikon representative