Enables the visualization of single molecule activity in living cells.
Nikon's Laser TIRF is a turnkey evanescent wave imaging system that's so user friendly, it's as simple to use as a standard fluorescence microscope. The evanescent wave resulting from total internal reflection (TIR) causes excitation of fluorescent molecules in an optical section, typically less than 100nm, without exciting molecules throughout the specimen. Evanescent wave illumination provides such high signal to noise (S/N) ratios that it is possible to observe single molecule activity in living cells where they are in contact with the coverglass.
