SRIC | SRIC

Parole chiave: white-light TIRF, TIRF

Definizione:SRIC is used in TIRF imaging to confirm that areas of a specimen are in contact with the coverslip or culture vessel

TECHNOLOGY:

In TIRF microscopy, illumination excites fluorophores within approximately 100 nm of the glass surface (coverslip or culture vessel). Specimens, therefore, must adhere to the glass surface for TIRF imaging to occur. In SRIC imaging, areas of the specimen in contact with the glass appear black, allowing users to confirm whether and where contact occurs. SRIC does not involve fluorophore excitation so phototoxicity and specimen damage are minimized. Users can ensure that all imaging criteria are correct (focus, contact) before proceeding with TIRF.

APPLICATION:

SRIC is applicable to all TIRF imaging modes (laser TIRF and white-light TIRF) for identifying the basal surface of the cell. It requires a modification to the epi-fluorescent attachment

MICROSCOPE CONFIGURATION:

SRIC can be used with both Nikon's laser TIRF and white-light TIRF systems using the Eclipse TE2000 series of inverted microscopes. The modular structure of these microscopes allows a modification to the epi-fluorescence attachment to allow SRIC imaging. SRIC simply involves a switch in imaging mode. TIRF and epi-fluoresence / SIRC can be mounted simultaneously with no restriction on their individual capabilities.

SYSTEM SOLUTION:

Nikon's TE2000-E inverted microscope configured for TIRF imaging together with the C1 for full flexibility for switching between confocal imaging and TIRF imaging.

FURTHER INFORMATION: