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Aberración cromática | Aberración cromática

Key Words: aberración esférica, Resolución, AN, aberración cromática (lateral), brillo

Definition:La inhabilidad de un lente de objetivo para traer luz de colores diferentes (longitudes de ondas diferentes) al mismo plano de enfoque.

TECHNOLOGY:

Light of different wavelengths from a specimen are refracted to different degrees as they pass through different media - e.g. from air to glass in the objective lens. Not all wavelengths, therefore, come together at the same point of focus. Blue light focuses at a point nearer the lens than green and red light resulting in color artefacts in the image - typically in the form of a purple halo around a brighter focused spot.

Certain types of objective lenses, using a combination of different types of glass with different refraction and dispersion characteristics, correct chromatic aberrations by ensuring that different wavelengths focus at the same plane. An Achromat objective focuses blue (about 486 nm - also called the 'f' line) and red (656 nm - the 'c' line). A Plan Achromat additionally correct in the 'g' line (436 nm) and Plan Fluor lenses also correct for green (588 nm - 'd' line). Plan Apochromat violet-corrected (VC) objectives additionally cover the 'h' line (405 nm) - totally correcting for five lines ranging from 405 - 656 nm.

APPLICATIONS:

The selection of appropriate lenses is critical when setting up a new imaging applications, digital imaging system or confocal microscopy system. Optical aberrations may have a particularly significant negative effect on the quality of confocal imaging. Chromatic aberrations interfere with image resolution and may lead to the misinterpretation of images

Caption: Improvement in chromatic aberration with Plan Apochromat violet-corrected (VC) objective.

MICROSCOPE CONFIGURATION:

Nikon's CFI60 objectives are easily interchangeable on Nikon's microscopes allowing the optimal objective to be selected for each application. Most laser scanning confocal microscopes use blue (488 nm), green (543 nm) or red (633 nm) excitation. At these wavelengths correction and transmission is optimal in Plan Fluor and Plan Apochromat series lenses. Certain probes require Plan Apochromat VC optics for activation and imaging including CFP, GFP, YFP, Cherry-FP, Tomato-FP, PA-GFP, Kaede, and PS-CFP. Plan Apochromat VC lenses not only work well in the violet part of the spectrum, but also perform well in the IR enabling greater depth penetration in the specimen.

RECOMMENDED SYSTEM:

Nikon's Plan Apochromat (VC) series is chromatically corrected for five lines ranging from 405 - 656 nm. This lens is also vignetting-free - the resolution of the objective lens is even across the entire field of view making it an ideal choice for digital photomicrography.

LINKS:

Chromatic aberration

Chromatic aberration and confocal microscopy

Introduction to objectives

Specialized microscope objectives


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