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Nikon Instruments / Products / Microscope Systems / Confocal Microscopes / A1R+ A1+ Confocal

Nikon A1R-A1 Confocal Microscope System

A1R+-A1+ Specifications

Input/Output Port 2 laser input ports
4 signal output ports for 4-PMT detector, spectral detector, VAAS (optional), and third-party detector (FCS/FCCS/FLIM)
Laser
Compatible Laser 405 nm, 440/445 nm, 488 nm, 561/594 nm, 638/640 nm, Ar laser (457 nm, 488 nm, 514 nm), HeNe laser (543 nm)
Modulation Method: AOTF (Acousto-Optic Tunable Filter) or AOM (Acousto-Optic Modulator) device
Control: power control for each wavelength, return mask, ROI exposure control
Laser Unit Standard: LU4A 4-laser module A or C-LU3EX 3-laser module EX
Optional: C-LU3EX 3-laser module EX (when 4-laser module A is chosen as standard laser unit)
Standard Fluorescence Detector
Wavelength 400-750 nm
Detector 4 PMT
Filter Cube 6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75
Diascopic Detector (option)
Wavelength 450-650 nm
Detector PMT
   
FOV Square inscribed in a o18 mm circle
Image Bit Depth 4096 gray intensity levels (12 bit)
Scanning Head
Standard Image Acquisition Scanner: galvano scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed:
Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction)
Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*1
Zoom: 1-1000x continuously variable
Scanning mode: X-Y, X-T, X-Z, XY rotation, Free line
High-Speed Image Acquisition (A1R+) Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis)
Pixel size: max. 512 x 512 pixels
Scanning speed: 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed)
Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Scanning mode: X-Y, X-T, X-Z
Acquisition method: Standard image acquisition, High-speed image acquisition, Simultaneous photoactivation and image acquisition
Dichroic Mirror Low-angle incidence method, Position: 8
Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80
Optional filter: 457, 405/488/543, 457/514/561, 440/514/594
Pinhole 12-256 µm variable (1st image plane)
Spectral Detector (with Galvano Scanner) (option)
Number of Channels 32 channels
Wavelength Detection Range 400-750 nm
Spectral Image Acquisition Speed 4 fps (256 x 256 pixels), 1000 lps
Pixel size: max. 2048 x 2048
Wavelength and Resolution 80 nm (2.5 nm), 192 nm (6 nm), 320 nm (10 nm)
Wavelength range variable in 0.25 nm steps
Unmixing High-speed unmixing, Precision unmixing
Z step Ti-E: 0.025 μm, FN1 stepping motor: 0.05 μm
Ni-E: 0.025 μm
Compatible Microscopes

ECLIPSE Ti-E inverted microscope

ECLIPSE FN1 fixed stage microscope

ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type)

Option

Motorized XY stage (for Ti-E/Ni-E), High-speed Z stage (for Ti-E)

High-speed piezo objective-positioning system (for FN1/Ni-E), VAAS

Software
Display/Image Generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Image Format JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS
Applications FRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control Computer
OS Microsoft Windows 7 Professional 64bits SP1 (Japanese version / English version)
CPU Intel Xeon X5672 (3.20 GHz/8 MB/1333 MHz/Quad Core) or higher
Memory 12 GB (2 GB x 3 + additional 2 GB x 3)
Hard Disk 300 GB SAS (15,000 rpm) x2, RAID 0 configuration
Data Transfer Dedicated data transfer I/F
Network Interface 10/100/1000 Gigabit Ethernet
Monitor 1600 x 1200 or higher resolution, dual monitor configuration recommended
   
Recommended Installation Conditions Temperature 23 ± 5 oC, humidity 60 % (RH) or less (non-condensing)