Cookies on the Nikon Instruments website
We use cookies to ensure we give you the best experience on our website. If you continue we'll assume
that you are happy to receive all cookies on the Nikon Instruments website.
Find out more about our cookie policy

Nikon Instruments / Products / Microscope Systems / Confocal Microscopes / A1R+ A1+ Confocal

Nikon A1R-A1 Confocal Microscope System

A1R+-A1+ Specifications

Input/Output Port 2 laser input ports
4 signal output ports for 4-PMT detector, spectral detector, VAAS (optional), and third-party detector (FCS/FCCS/FLIM)
Compatible Laser 405 nm, 440/445 nm, 488 nm, 561/594 nm, 638/640 nm, Ar laser (457 nm, 488 nm, 514 nm), HeNe laser (543 nm)
Modulation Method: AOTF (Acousto-Optic Tunable Filter) or AOM (Acousto-Optic Modulator) device
Control: power control for each wavelength, return mask, ROI exposure control
Laser Unit Standard: LU4A 4-laser module A or C-LU3EX 3-laser module EX
Optional: C-LU3EX 3-laser module EX (when 4-laser module A is chosen as standard laser unit)
Standard Fluorescence Detector
Wavelength 400-750 nm
Detector 4 PMT
Filter Cube 6 filter cubes commonly used for a microscope mountable on each of three filter wheels
Recommended wavelengths: 450/50, 482/35, 515/30, 525/50, 540/30, 550/49, 585/65, 595/50, 700/75
Diascopic Detector (option)
Wavelength 450-650 nm
Detector PMT
FOV Square inscribed in a o18 mm circle
Image Bit Depth 4096 gray intensity levels (12 bit)
Scanning Head
Standard Image Acquisition Scanner: galvano scanner x2
Pixel size: max. 4096 x 4096 pixels
Scanning speed:
Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction)
Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*1
Zoom: 1-1000x continuously variable
Scanning mode: X-Y, X-T, X-Z, XY rotation, Free line
High-Speed Image Acquisition (A1R+) Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis)
Pixel size: max. 512 x 512 pixels
Scanning speed: 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed)
Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Scanning mode: X-Y, X-T, X-Z
Acquisition method: Standard image acquisition, High-speed image acquisition, Simultaneous photoactivation and image acquisition
Dichroic Mirror Low-angle incidence method, Position: 8
Standard filter: 405/488, 405/488/561, 405/488/561/638, 405/488/543/638, 457/514, BS20/80
Optional filter: 457, 405/488/543, 457/514/561, 440/514/594
Pinhole 12-256 µm variable (1st image plane)
Spectral Detector (with Galvano Scanner) (option)
Number of Channels 32 channels
Wavelength Detection Range 400-750 nm
Spectral Image Acquisition Speed 4 fps (256 x 256 pixels), 1000 lps
Pixel size: max. 2048 x 2048
Wavelength and Resolution 80 nm (2.5 nm), 192 nm (6 nm), 320 nm (10 nm)
Wavelength range variable in 0.25 nm steps
Unmixing High-speed unmixing, Precision unmixing
Z step Ti-E: 0.025 μm, FN1 stepping motor: 0.05 μm
Ni-E: 0.025 μm
Compatible Microscopes

ECLIPSE Ti-E inverted microscope

ECLIPSE FN1 fixed stage microscope

ECLIPSE Ni-E upright microscope (focusing nosepiece type and focusing stage type)


Motorized XY stage (for Ti-E/Ni-E), High-speed Z stage (for Ti-E)

High-speed piezo objective-positioning system (for FN1/Ni-E), VAAS

Display/Image Generation 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
Applications FRAP, FLIP, FRET (option), photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
Control Computer
OS Microsoft Windows 7 Professional 64bits SP1 (Japanese version / English version)
CPU Intel Xeon X5672 (3.20 GHz/8 MB/1333 MHz/Quad Core) or higher
Memory 12 GB (2 GB x 3 + additional 2 GB x 3)
Hard Disk 300 GB SAS (15,000 rpm) x2, RAID 0 configuration
Data Transfer Dedicated data transfer I/F
Network Interface 10/100/1000 Gigabit Ethernet
Monitor 1600 x 1200 or higher resolution, dual monitor configuration recommended
Recommended Installation Conditions Temperature 23 ± 5 oC, humidity 60 % (RH) or less (non-condensing)