N-SIM Specifications
| Super-Resolution |
Lateral (XY): ~85-110nm (dependant on wavelength and optics) Axial (Z): ~200-250nm (dependant on wavelength and optics) 3D Axial Range: up to 20μm |
|---|---|
| Speed |
TIRF and 2D SIM = 0.6 sec/frame (dependent on exposure time) 3D SIM = 1.0 sec/frame (dependent on exposure time) |
| Microscope |
Ti-E with Perfect Focus System and SIM Illuminator SIM Microscope Enclosure |
| Objectives |
Apo TIRF 100X 1.49 Oil Plan Apo IR 60X 1.27 Water Immersion |
| Available Lasers, AOTF Modulated |
457nm (100mW) 488nm (200mW) 515nm (100mW) 532nm (100mW) 561nm (100mW) |
| Camera | EM-CCD Camera iXon DU897 (Andor) |
| Anti-Vibration Optical Table | Required |
| Operating Temperature | 25°C ± 0.5°C |
| Operating Humidity | 40-50% ± 3% |
System Diagram
