A1R MP Specifications
| Input/output Port |
3 laser input ports 4 signal output ports (for 4-PMT detector, spectral detector, VAAS, optional detector*1) |
|---|---|
| Laser for Confocal Microscopy | |
| Compatible Laser | Laser diode (405 nm, max. 36mW), Laser diode (440 nm, max. 20mW), Ar laser (457 nm/478 nm*2/514 nm, max. 65 mW), Solid-state laser (488 nm, max. 20mW), Solid-state laser (561 nm, max. 25mW), G-HeNe laser (543 nm, max. 1 mW), Laser diode (638 nm, max. 10mW) |
| Modulation |
Method: AOTF (Acousto-Optical Tunable Filter) or AOM (Acousto-Optical Modulator) device Control: power control for each wavelength, return mask, ROI exposure control |
| Laser Unit |
Standard: 4-laser module or 3-laser module EX Optional: 3-laser module EX (when 4-laser module is chosen as standard laser unit) |
| Laser for Multiphoton Microscopy | |
| Compatible Laser |
Mai Tai HP DeepSee (Newport Corp.) Chameleon Vision II (Coherent Inc.) |
| Modulation |
Method: AOM (Acousto-Optical Modulator) device Control: power control, return mask, ROI exposure control |
| Incident Optics | 700-1000 nm, auto alignment |
| Standard 4-Channel Detector | |
| Wavelength | 400-750 nm (400-650 nm for multiphoton observation) |
| Detector | 4 PMT |
| Filter Cube |
6 filter cubes commonly used for a microscope mountable on each of three filter wheels Recommended wavelengths for multiphoton/confocal observation: 450/50, 482/35, 515/30, 525/50, 540/30, 585/65, 595/50, 700/75 |
| Diascopic Detector | |
| Wavelength | 440-645 nm |
| Detector | PMT |
| NDD for Multiphoton Microscopy | |
| Wavelength | 400-650 nm |
| Detector | 4 PMT |
| Filter Cube |
Filter cubes commonly used for a microscope Recommended filter sets for multiphoton: 492SP, 525/50, 575/25, 692/53, DM458, DM495, DM511, DM560, DM593 |
| Unmixing | Channel unmixing |
| Image Bit Depth | 4096 gray intensity levels (12 bit) |
| Scanning Head | |
| Scanning |
FOV: Square inscribed in a ∅18 mm circle Standard image acquisition Scanner: non-resonant scanner x2 Pixel size: max. 4096 x 4096 pixels Scanning speed: standard 1 fps (512 x 512 pixels), max. 4 fps (512 x 512 pixels) Zoom: 1-1000x continuously variable Scanning mode: X-Y, XY rotation, Free line*3, Line Z High-speed image acquisition Scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), non-resonant scanner (Y-axis) Pixel size: max. 512 x 512 pixels Scanning speed: 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed) Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x) Scanning mode: X-Y, Line Acquisition method: Standard image acquisition, High-speed image acquisition, Simultaneous photo activation and image acquisition*4 |
| Dichroic Mirror |
Low-angle incidence method Position: 8 Standard filter: 405/488, 405/488/561, 405/488/561/638, 457/514, 405/488/543/638, BS20/80, IR, 405/488/561/IR |
| Pinhole | 12-256 μm variable (1st image plane) |
| Wavelength Detection Range | 400 nm-750 nm (400 nm-650 nm with multiphoton microscopy) |
| Number of Channels | 32 channels |
| Spectral Image Acquisition Speed | 4 fps (256 x 256 pixels), 1000 lps |
| Wavelength Resolution |
2.5 nm, 6 nm, 10 nm Wavelength range variable in 0.25 nm steps |
| Unmixing | High-speed unmixing, Precision unmixing |
| Compatible Microscopes |
ECLIPSE Ti-E inverted microscope, ECLIPSE FN1 fixed stage microscope Z step |
| Z Step | Ti-E: 0.025 μm, FN1 stepping motor: 0.05 μm |
| Option | Motorized XY stage (for Ti-E), High-speed Z stage (for Ti-E), High-speed piezo objective-positioning system (for FN1), VAAS |
| Software | |
| Display/Image Generation | 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing |
| Image Format | JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS |
| Application | FRAP, FLIP, FRET, photo activation*4, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization |
| OS | Microsoft Windows® Vista Business 64-bit SP1 (Japanese version /English version) |
| CPU | Intel Xeon X5570 (2.98 GHz/8 MB/1333 MHz) |
| Memory | 12 GB |
| Hard Disk | SAS (15,000 rpm), 300 GB x2, RAID 0 configuration |
| Data Transfer | Dedicated data transfer I/F |
| Monitor | 1600 x 1200 or higher resolution, dual monitor configuration recommended |
| Vibration Isolated Table | 1800 (W) x 1500 (D) mm recommended, or 1500 (W) x 1500 (D) mm |
