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Super-Resolution Microscope System

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Ten times the resolution of conventional optical microscopes

N-STORM is a super-resolution microscope system that combines “STochastic Optical Reconstruction Microscopy” technology (licensed from Harvard University) and Nikon's Eclipse Ti research inverted microscope. The N-STORM super-resolution microscope provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes.

Key Features

Ten Times the Resolution of Conventional Optical Microscopes

N-STORM utilizes a high accuracy localization information for thousands of individual fluorophores present in a field of view to create breathtaking super-resolution images exhibiting spatial resolution of 20nm; ten times greater than conventional optical microscopes.

N-STORM_resolution

3D Imaging with 50 nm Axial Resolution

In addition to lateral super-resolution, N-STORM utilizes proprietary methods to achieve a tenfold enhancement in axial resolution to 50nm, effectively providing 3D information at a nanoscopic scale.

Fluorescence labeled microtubule

3D-STORM image of antibody-labeled microtubules. Colors encode z-depth information.


Unique Multi-color Technology

Multi-color super-resolution imaging can be carried out using several dye strategies. Unique tandem dye pairs that combine "activator" and "reporter" probes allow highly accurate localization with no z shift.


Continuous Activation Imaging with a Single Dye

An alternative dye strategy is to use standard commercially available secondary antibodies for continuous activation imaging to easily gain critical insights into the localization of multiple proteins at the molecular level.

N-STORM_continuous_activation

Combination with A1R+

N_STORM_A1R

The N-STORM system is based around the Nikon Ti-E inverted microscope. This flexible platform allows combination with confocals such as the A1R+ for live-cell imaging, low magnification observation, photo-stimulation and 20nm-resolution imaging on one integrated system, under the control of the universal NIS-Elements platform.


Combination with N-SIM

N_STORM_N-SIM

N-SIM and N-STORM can be combined on a single Nikon Ti-E inverted microscope to create the ultimate super-resolution imaging system. Using the N-SIM/N-STORM kit, switching between live cell super-resolution and 20nm-resolution imaging is possible without having to change the camera adapter.


Fit Overlapping Peaks Function

  • Identifies and fits more molecules per frame
  • Works for densely labeled samples
  • Increases reconstruction localization density
  • Can be used for 2D and 3D STORM data sets
  • Based on a published algorithm (Babcock et al., 2012. Optical Nanoscopy. 1:6)
FIT1
Original Data
FIT2
Detected Molecules with
Conventional Fitting
FIT3
Detected Molecules with
Fit Overlapping Peaks
351,560 mol.
FIT4
Without FOP
1,065,088 mol.
FIT5
With FOP


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