Super-resolution microscope visualizing cellular structures and molecular activity at resolutions never before achieved by conventional light microscopy.
Nikon’s N-SIM microscopy system can produce twice the resolution of conventional optical microscopes. Using an innovative approach based on Structured Illumination Microscopy technology licensed from UCSF, the N-SIM enables detailed visualization of minute intracellular structures and their functions.
Twice the Resolution of Conventional Optical Microscopes
N-SIM doubles the resolution of conventional optical microscopes (~115nm in 3D SIM mode*) by combining “Structured Illumination Microscopy” technology licensed from UCSF with Nikon’s renowned CFI Apo TIRF 100x oil objective lens (N.A. 1.49).
* resolution will depend on laser wavelength and imaging mode.
Dedicated Super-resolution Objectives
The SR (super-resolution) objectives have been designed for new applications that break the diffraction barrier.
The most recent optical designs using wavefront aberration measurement have been applied to yield superb optical performances with the lowest asymmetric aberration.
Time Resolution of 0.6 sec/frame, the Fastest in the Industry
N-SIM provides the fastest imaging capability in the industry, with a time resolution of 0.6 sec/frame, effective for live-cell imaging.
Live Cell Super-Resolution Imaging
The combination of fast image capture and the availability of stage top incubation allows live cell imaging at twice the resolution of conventional microscopes.
Multiple SIM Modes
The 3D-SIM illumination technique improves axial resolution to 269 nm and has the capability of optical sectioning of specimens, enabling the visualization of more detailed cell structures at higher spacial resolutions.
This mode captures super-resolution 2D images at high speed with incredible contrast. TIRF-SIM mode takes advantage of Total Internal Reflection Fluorescence observation at double the resolution as compared to conventional TIRF microscopes, facilitating a greater understanding of molecular interactions at the cell surface.
Plasma membrane of B16 melanoma cell labeled with YFP
Objective: CFI Apochromat TIRF 100x oil (NA 1.49)
Photographed with the cooperation of: Dr. Yasushi Okada, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN
5 Laser Multi-color Super-resolution Imaging
The Nikon N-SIM system allows multi-channel imaging with up to 5 lasers enabling the study of dynamic interactions of multiple proteins of interest at the molecular level.
Simultaneous Two-wavelength Super-resolution Imaging
By attaching two EMCCD cameras to the microscope with the optional Two Camera Imaging Adapter*, simultaneous two-wavelength super-resolution imaging with excitation of 488 nm and 561 nm is possible.
* Andor Technology Ltd.