Ten times the resolution of conventional optical microscopes
N-STORM is a super-resolution microscope system that combines “STochastic Optical Reconstruction Microscopy” technology (licensed from Harvard University) and Nikon's Eclipse Ti research inverted microscope. The N-STORM super-resolution microscope provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes.
Ten Times the Resolution of Conventional Optical Microscopes
N-STORM utilizes a high accuracy localization information for thousands of individual fluorophores present in a field of view to create breathtaking super-resolution images exhibiting spatial resolution of 20nm; ten times greater than conventional optical microscopes.
3D Imaging with 50 nm Axial Resolution
In addition to lateral super-resolution, N-STORM utilizes proprietary methods to achieve a tenfold enhancement in axial resolution to 50nm, effectively providing 3D information at a nanoscopic scale.
Unique Multi-color Technology
Multi-color super-resolution imaging can be carried out using several dye strategies.
Unique tandem dye pairs (e.g. Alexa 405-Alexa 647, Cy2-Alexa 647, Cy3-Alexa 647) that combine "activator" and "reporter" probes allow highly accurate localization with no z shift.
Continuous Activation Imaging with a Single Dye
An alternative dye strategy is to use standard commercially available secondary antibodies for continuous activation imaging to easily gain critical insights into the localization of multiple proteins at the molecular level.
Combination with A1R+
The N-STORM system is based around the Nikon Ti-E inverted microscope. This flexible platform allows combination with confocals such as the A1R+ for live-cell imaging, low magnification observation, photo-stimulation and 20nm-resolution imaging on one integrated system, under the control of the universal NIS-Elements platform.
Combination with N-SIM
N-SIM and N-STORM can be combined on a single Nikon Ti-E inverted microscope to create the ultimate super-resolution imaging system. Using the N-SIM/N-STORM kit, switching between live cell super-resolution and 20nm-resolution imaging is possible without having to change the camera adapter.
Image Construction by Overlaying Single Molecule Images
“STORM” is a new technology that reconstructs high resolution fluorescence images (2D or 3D) from localization information of fluorophores detected with high accuracy and calculated from multiple exposures. This technique generates much more detailed information and goes one step further, from structural to molecular understanding of the specimen.
Fit Overlapping Peaks Function
- Identifies and fits more molecules per frame
- Works for densely labeled samples
- Increases reconstruction localization density
- Can be used for 2D and 3D STORM data sets
- Based on a published algorithm (Babcock et al., 2012. Optical Nanoscopy. 1:6)
Detected Molecules with
Detected Molecules with
Fit Overlapping Peaks