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Inverted Microscope System

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Market leading inverted microscope capable of taking on the most advanced bioscience imaging protocols.

Scientists have overcome many live cell imaging challenges using advanced techniques such as TIRF, confocal, FRET, photoactivation and microinjection. At the center of it all is the Eclipse Ti-E, a powerful inverted microscope system that provides instant access to all of these techniques plus revolutionary Nikon CFI60 optics. The Eclipse Ti-E offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live cell imaging.

Key Features

Stable Time-lapse Imaging with Automatic Focus Correction System

PFS diagram using an immersion objective. A dry type objective is also available.

The Ti-E is equipped with a unique Perfect Focus System (PFS) that automatically corrects focus drift in real time during a prolonged period of time-lapse imaging.

Focus drift resulting from a temperature drop when reagents are added is instantaneously corrected and the rapid change of cells can be captured.

The incorporation of the PFS in the nosepiece unit saves space and allows two optical component levels to be attached simultaneously utilizing stratum structure.


Improved Performance in Broader Wavelength Range

By employing the 870nm wavelength for the coverglass interface detection, near-infrared fluorescence dyes including Cy5.5 can be used. Nikon offers two PFS models, one for UV-visible wavelength imaging and one for multiphoton imaging. The multiphoton model can correct for focus drift even when imaging with wavelengths ranging from 880-1300 nm.


Maintaining Focus at Greater Depths

Focus drift can now be corrected in a broader range of Z-axis planes than ever before. Maintaining focus at greater distances from the objective lens and at greater depths within the specimen is also possible.

In addition, PFS's focus drift correction range has been widened, resulting in more reliable and stable data.

Because PFS can maintain focus at greater depths within the specimen, whole images of intersegmental vessels sprouting upward from the dorsal aorta are clearly captured. Shown in the three channels are three different timepoint volumes (red: 0 mins, green: 110 mins, blue: 240 mins).

Specimen: vasculature of a zebrafish embryo (95-186 µm away from the coverslip).
Movie courtesy of: Dr. Robert Fischer, Marine Biological Laboratory


Compatible with Plastic Dishes and Well Plates

In addition to glass bottom dishes, plastic dishes can be used with PFS. The system is especially suited to high-throughput screening applications that involve multi-well plates.


High-speed Motorized Control and Acquisition

Multipoint snapshots of HeLa cells transiently expressing Venus-tubulin and mCherry-actin and stained with Hoechst33342 and DiD. (All in pseudo-color) Dr. Kenta Saito, Research Institute for Electronic Science, Hokkaido University and Dr. Takeharu Nagai, The Institute of Scientific and Industrial Research, Osaka University

The operational speeds of motorized components such as the nosepiece, fluorescence filters and stage have been greatly enhanced, allowing high-speed screening image capture during multi-dimensional experiments. Faster device movement and image acquisition reduce overall light exposure and subsequent photo-toxicity, leading to more meaningful data. The digital Controller Hub significantly increases motorized accessory speed by reducing communication overhead time between components, boosting total operation speed.


High-quality Phase Contrast Images using High NA Lenses

Phase Contrast

The revolutionary external phase contrast unit incorporates a phase ring and allows the use of high NA objective lenses without a phase ring for phase contrast observation. Because there is no light loss due to a phase ring, bright "full intensity" fluorescence images as well as high-resolution phase contrast images can be captured using the same objective lens.


Advanced Integration with Peripherals via NIS-Elements Imaging Software

Eclipse Ti-E 6D time-lapse Imaging System

Nikon's comprehensive imaging software NIS-Elements provides an integrated control of the microscope, cameras, components and peripherals. The intuitive GUI and efficient workflow make the programming of automated imaging sequences up to 6D (X, Y, Z, time, wavelength, multipoint) easy to perform.


Ti-LAPP Modular Illumination System

The new Ti-LAPP system provides a wide range of illumination modules that can be flexibly combined to create an imaging system tailored for your research. The modularity of the Ti-LAPP system also provides flexibility when the system configuration needs to be changed, an important feature in core imaging facilities and labs that have changing imaging needs.


DMD Module

The DMD module enables photoactivation and photoconversion of a user-specified pattern and position(s), whereas the conventional FRAP unit only enables photoactivation of a single, manually-positioned spot.

DMD Module

A mouse embryonic fibroblast co-expressing mCherry-tagged lamin A (red) and photo-activatable GFP-tagged lamin A was photo-converted (green) in the lower right region using the DMD module and 405 nm LED light. Time-lapse images were captured using the epi-fluorescence illuminator. By photoactivating a sub-population of the lamin proteins, one can observe their dynamics and subunit-exchange behavior.

Image courtesy of Drs. Takeshi Shimi and Bob Goldman, Northwestern University Medical School


H-TIRF Module

H-TIRF

Three-color TIRF image Using the gradation ND filter, a very even TIRF illumination is achieved. An in vitro preparation of fluorescently-labeled microtubules (tetramethylrhodamine and Alexa 647) and tubulin binding proteins (Alexa 488) was imaged using the H-TIRF illuminator and the gradation ND filter. Incident angles can be automatically adjusted for multiple wavelengths.

Image courtesy of Melissa Hendershott and Dr. Ron Vale, University of California, San Francisco

The H-TIRF module automatically adjusts the focus and incident angle of the laser for TIRF observation by monitoring the reflection beam. An even field of TIRF illumination is achieved with a gradation neutral density (ND) filter.


Flexible Module Combination

The Ti-Lapp system’s modularity and flexible configuration capability provide custom imaging solutions for individual research needs. Modules can also be easily exchanged or added to adapt to changing experimental needs, an important feature for labs with evolving research directions and multi-user, core facilities. For example, by adding a second TIRF module to a single-TIRF configuration, users can easily carry out anisotropy experiments and fast, multi-angle TIRF experiments. Adding a photoactivation/conversion module such as the DMD or FRAP module enables tracking of a sub-fraction of a protein population, providing insights into protein behaviors that would otherwise be illusive when imaging the entire population.

Flexible Module Combination

Two-tiered Configuration Capability

Taking advantage of the Nikon Ti’s stratum structure, modules can be incorporated as two separate layers with multiple modules per layer. Using a dual layer configuration enables optimal filter configuration for each illumination module. This enables optimal filter selection and improves experimental accuracy whilst maintaining the highest acquisition speeds.

Two-tiered configuration

Multiport Design with a Maximum of Five Imaging Ports

Multiport Design

Use of an optional back port enables multiple wavelength FRET imaging with multiple cameras. Moreover, by adding an eyepiece tube base unit with a side port, a maximum of five imaging ports* including left, right and bottom ports are available.

(*With Ti-E/B model with bottom port)



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