Bring imaging to life: high-speed, high-resolution confocal imaging.
Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, Nikon's A1R+ is a powerful tool for the imaging and visualization of intracellular dynamics and interaction.
Revolutionary Hybrid Confocal Scan Head
The A1R+ has a hybrid scanner head that incorporates both an ultrahigh-speed resonant scanner and a high-resolution galvano scanner. Simultaneous photoactivation and ultrafast imaging using these two scanners allow acquisition of rapid changes after photoactivation and enables observation of intermolecular interaction.
Resonant Scanner Provides Ultrafast Imaging
The A1R+ resonant scanner has an ultrahigh resonance frequency of 7.8 kHz. It allows imaging of intercellular dynamics at 30 fps (512 x 512 pixels) and 420 fps (512 x 32 pixels), the world's fastest image acquisition. The field of view of the scanned area is approximately five times larger than that of the galvano scanner. The Nikon original optical clock generation method realizes high image quality even at the highest speed. The fiber-optic communication data transfer system can transfer data at a maximum of 4Gbps.
Galvano Scanner Enables High-resolution Imaging
The A1+ utilizes a galvano scanner which enables high-resolution imaging of up to 4096 x 4096 pixels. In addition, with the newly developed scanner driving and sampling systems, plus image correction technology, high-speed acquisition of 10 fps (512 x 512 pixels) is also possible.
GaAsP Multi Detector Unit
A GaAsP PMT has much higher sensitivity than a normal PMT, thus acquisition of brighter signals with minimal background noise is possible with a GaAsP PMT, even with weak fluorescence, which, until now, has been difficult to detect.
When using resonant scanners, the GaAsP PMT enables low-noise, high-speed imaging.
Increased Light Detection Efficiency
incidence angle method
Reflection-transmission characteristics have high polarization dependence
Reflection-transmission characteristics have lower polarization dependence
By employing the hexagonal pinhole, higher brightness equivalent to that of a circular pinhole is achieved.
64% of the area of the circle
30% more light||
83% of the area of the circle
Nikon's original dual integration signal processing technology (DISP) has been implemented in the image processing circuitry to improve electrical efficiency, resulting in an extremely high S/N ratio.
Enhanced Spectral Imaging
Accurate, High-speed Unmixing
Accurate spectral unmixing provides maximum performance in the separation of closely overlapping fluorescence spectra and the elimination of autofluorescence. Superior algorithms and high-speed data processing enable real time unmixing during image acquisition.
Filter-less intensity adjustment is possible by selecting desired spectral ranges from 32 channels that match the spectrum of the fluorescence probe in use and combining them to perform the filtering function.
Increased flexibility and ease of use
NIS-Elements C control software enables integrated control of the confocal imaging system, microscope and peripheral devices with a simple and intuitive interface. Diverse reliable analysis functions are also available.