Mercury Fibre Illumination | Mercury Fibre Illumination
Key Words: Fluorescence, illumination, epi-fluorescence, reflected light
Definition:A mercury microscope illuminator for epi-fluorescence with optical fiber connection that avoids transfer of heat or electrical noise to the microscope body
TECHNOLOGY:
Nikon’s Intensilight mercury-fiber microscope illumination system for epi-fluorescence applications provides no-flicker, stable light intensity which can be controlled with Nikon's NIS-Elements software. Stable light intensity that completely fills the back aperture is obtained through the use of direct current instead of an alternating current power source. Typical lamp lifetimes are in the region of 2000 hours as compared to conventional mercury lamps (approximately 200 hours). The illumination is pre-centred and provides easy lamp replacement: no alignment is required.
APPLICATIONS:
The Intensilight is suitable for epi-fluorescence applications in both biological and industrial applications. It is suitable for quantitative fluorescence and has high transmission at lower UV wavelengths. Six levels of light intensity allow users to modulate illumination, therefore enabling reduced photobleaching of fluorophores and reduced phototoxicity to living cells while insuring shading free and extremely even specimen illumination. The Intensilight also delivers consistent reflected light, useful in industrial microscope applications.
MICROSCOPE CONFIGURATION:
Nikon's Intensilight includes matched adapters for different families of Nikon microscopes. A built in open-close shutter makes it easy to transfer the illumination source to multiple microscopes or to shutter the illuminator's light to the microscope specimen. Two sizes of liquid light guide fibers in lengths of 1500mm or 3000mm are available. The Intensilight system includes two models, a manual version with manual shutter and neutral density selector controls, and a motorized and PC controllable version that has an optional desk top control pad for standard use
RECOMMENDED SYSTEM:
Please consult your Nikon representative for further advice on illumination systems.
LINKS:
Introduction to fluorescence microscopy [microscopyu]
Epi-fluorescence for stereomicroscopy [microscopyu]
