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Nikon's A1R MP is an advanced multiphoton excitation and confocal fluorescence imaging system that features a high resolution galvanometer scanner and a high speed resonance scanner capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode. New 4 channel non-descanned detectors with higher sensitivity, reduced dark current, and broad spectral range allow for real-time unmixing of closely spaced probes for accurate and high-contrast spectral imaging; which is so important in multiphoton imaging because of the overlap of emission spectra of probes and autofluorescence often unavoidable when a single laser line is used. The Nikon A1R MP also features a newly developed one click auto-alignment of multiphoton excitation laser allowing for fast set-up, wavelength changes, GVD compensation and total ease of use. This device also completely encloses the beam within the instrument from the laser to the objective lens (a huge improvement in the operating safety of multiphoton imaging systems).

• Cell Friendly - Fast Resonant Imaging at up to 420 FPS
• NDDs positioned for maximum fluorescence efficiency
• New Lambda-S Objective Lenses feature the highest NA’s in their class
• Incident Optical Unit and auto-aligns the laser when changing objectives
• NDD Virtual Channel unmixing with single MP excitation

Nikon's new objectives offer both high NA and long working distance in addition to high transmission made possible by Nikon's exclusive Nano Crystal Coat technology. Fine structures and motions inside a specimen are clearly observed with excellent resolution and brightness.

λS APO Objectives

• World’s highest N.A.’s for water immersion
• Highest transmission rates
• Highly corrected throughout broad-band
• Expanded spectral range
• Made possible by new exclusive Nano Crystal Coat

Nano Crystal Coatings

• Anti-Reflective coating of Nano Crystal particles
• Eliminates internal lens reflections and flare
• Employs multiple layers of extra-low refractive index Nano Crystals
• Increases S/N and transmission across wide wavelength range

The A1Rsi features the full integration of Nikon's A1 confocal microscope with the Ti Perfect Focus inverted microscope. A1-SHR dual scanning technology features high speed resonant image acquisition up to 420 FPS and up to 4096 x 4096 pixel resolution with flexible stimulation and simultaneous image acquisition. Laser units include AOTF modulated and AOM that provide up to 10 laser lines with a new 32-Channel Spectral Detector for high accuracy spectral acquisition. In conjunction with NIS-Elements, the platform is unified for an impressive suite of analysis tools for general to high end applications: Deconvolution, FRET, Colocalization, 2Tracking/Kymograph, a wide array of viewing and rendering tools, and powerful presentation and data output capabilities.

• A1R’s hybrid scanner provides ultra-high speed imaging and simultaneous photoactivation
• FAST 32 channel imaging with incredible sensitivity and virtual filtering capability
• Resonant scanning up to 420 FPS

The C1si opens a new world of true spectral imaging through enhanced sensitivity, variable spectral resolution at 2.5nm, 5nm or 10nm for true high spectral resolution, rapid real time and high accuracy unmixing options, and 32 channel simultaneous acquisition for fast one shot spectral capture.

• True “One Shot” 32 channel spectral imaging with accuracy and sensitivity
• 2.5nm, 5nm, or 10nm spectral resolution
• Amazing image quality, flexibility, and modularity

The Ti-E is the ideal imaging solution for long term live cell observations. This inverted microscope system is designed for speed with its UV, visible & IR PFS w/remote offset, high speed precision encoded XY stage & Piezo Z for 96 well plate and ability to achieve 3 channel FL + DIC high speed macro with true encoded Z to 25nm. NIS-Elements, Nikon’s premier imaging software, seamlessly integrates system components for optimal hardware performance and powerful quantitative analysis while providing flexible and innovative acquisition functions for multi-dimensional imaging. NIS-Elements creates an easy setup routine for multi-well plate acquisition: Multi-channel, multi-position per well, time-lapse and Z-Series- all in Perfect Focus.

• Built for SPEED capable of imaging a 96 Well Plate in 3 channels in less than 5 minutes
• Dynamic Content Rich Live Cell Imaging all in “Perfect Focus”
• Enabled by the POWER and ease of NIS-Elements multi-dimensional acquisition software

The Ti-E with multi-channel automated laser TIRF with new simultaneous Multi-Z imaging is Nikon's premier automated inverted microscope. Faster communication time between components reduces lag time between operations while integrating control and automation while Nikon’s real time continuous Perfect Focus correction system virtually eliminates focus drift. Also included are high NA Laser TIRF with 1.49 NA objectives for high resolution and True Simultaneous, Dual Camera Control for high speed, high resolution multi-channel acquisition.

• Simultaneous photo-activation and multi-spectral imaging of single molecules
• True simultaneous, dual camera control for high speed, high resolution multi-channel acquisition
• 1.49NA Apo TIRF optics

The Ti-U Transgenic Micromanipulation System is a full system solution for transgenic techniques and stem cell research through the use of Xenoworks micromanipulators and NAMC (Nikon Advanced Modulation Contrast), Nikon’s new light microscopy contrast method. The system offers enhanced and smooth manipulation with DIC type observation of specimens in plastic dishes.

• Ti-U Universal Inverted Microscope with left and right photoports, large vibration resistant main body for optimal image stability and smooth micromanipulation
• NAMC – Nikon Advanced Modulation Contrast – High numerical aperture. LWD objectives produce three-dimensional appearing images even in plastic. Objectives are also compatible with fluorescence microscopy.
• Xenoworks – smooth, precise, highly responsive micromanipulator for Zygote pronuclear injection, embryonic stem cell blastocyst transfer and adherent cell microinjection

The AZ100 Macro Imaging System is the best of a stereo and compound microscope combined into single hybrid system that allows dynamic imaging of single cells or whole specimens. The microscope features an on-axis optical system for high resolution imaging and long working distance for tall samples in order to achieve simultaneous fluorescence and DIC, 3D reconstruction and time-lapse.

• Broad magnification range enables imaging of specimens from single cells to whole animals
• On-axis optical system facilitates true macro DIC and accurate 3D reconstruction
• Imaging from 5x to 400x

Nikon's NIS-Elements revolutionizes imaging software for the microscopy market by combining automated intelligence to microscopes, cameras, components and peripherals with powerful archiving, analysis, visualization and archiving tools. Its intuitive interface simplifies workflow and speeds up image acquisition times while providing powerful features such as image stitching, object counting and volume views. NIS-Elements makes it possible to capture, display, analyze and export data generated from 6-D datasets. Volume and tiled views, image counts, live graphing and intensity information are available during acquisition; and, as the quantity and complexity of image datasets increase, NIS-Elements adds the necessary intuitive workflow and controls for data extraction, management and export.

The BioStation IM is a self-contained cell incubation and monitoring system that’s automated and easy to use. It maintains a cell friendly environment and reduces cell stress with no focus drift design making it ideal for long duration time-lapse imaging and optimizing stem cell differentiation protocols. The IM is a totally integrated system with temperature, humidity and CO2 control; internal motorized X, Y and Z for multi-point time-lapse acquisition; phase contrast and multi-channel fluorescence observation.

BioStation IM-Q

• All-in-one time-lapse fluorescence imaging
• Total environmental control
• Automated multipoint time-lapse
• Perfusion enabled

The Biostation CT is the world’s first integrated and automated cell culturing and observation system that images cells without removal from the incubation chamber. Ideal for identifying cellular health, proliferation rates, and pluripotency while minimizing the stress on cells and enabling simultaneous multiple users with multiple experiments. With integrated robotics and auto-focus, BioStation CT can handle up to 30 well plates or flasks. An image analysis suite quantitatively measures cellular events and is capable of high-content analysis.

• Total environmental control incubation
• Capacity of 30 well-plates or flasks
• Automated microscope with integrated robotics
• Multipoint time-lapse
• 3-channel LED-based fluorescence

CL-Quant Automated Image Analysis Software

• Label-free phase or fluorescence analysis
• Teachable interface for analysis creation
• Batch-processing for high-content screening