Cell Signaling

Fluorescence microscopes and especially confocal fluorescence imaging, optical sectioning and 3-D reconstruction are powerful techniques that allow researchers to look into cells and tissues to identify and locate molecules of interest in and between cells. Fluorescent probes such as the genetically encoded GFP family and chemical probes are central to the observation of molecules /ions of interest.

Live cell imaging allows signaling events to be observed directly and over extended periods of time, giving researchers temporal and spatial continuity in event monitoring. The development of the GFP family of fluorescent probes allows monitoring of protein expression, locating and tracking molecules and identification of intermolecular interactions. GFPs fused to a calcium binding protein can also be used to visualize calcium signaling events central to many cell communication events. Key imaging techniques include multiphoton uncaging, FRET, FLIM, TIRF, FRAP, FLIP and BRET. Multiple probes may be used simultaneously and identified with high resolution using spectral imaging capabilities.

Image Credit: Fixed Neuronal Cells of Mouse Brain Expressing eGFP. Dr Satoru Kondo. Department of Cellular Neurobiology, Graduate School of Medicine, University of Tokyo